1. |
Advances and Challenges in the Analysis of Boswellic Acids by Separation Methods.
Kosolapov D, Jáč P, Riasová P, Poušková J, Polášek M, Nováková L
resin is an exudate from the cut bark of trees. The main constituents of pharmacological interest are boswellic acids (pentacyclic triterpenoids), namely α-boswellic acid, β-boswellic acid, 3--acetyl-α-boswellic acid, 3-acetyl-β-boswellic acid, 11-keto-β-boswellic acid, and 3--acetyl-11-keto-β-boswellic acid. Nowadays, dietary supplements with extract are used in the treatment of inflammatory joint diseases. Additionally, the constituents of resin have shown potential for the treatment of other chronic inflammatory diseases and various types of cancer. Separation methods including ultra/high-performance liquid chromatography, gas chromatography, thin layer chromatography, supercritical fluid chromatography, and capillary electrochromatography coupled with UV or MS detection have been used for the determination of boswellic acids in various matrices (mostly plant material and biological samples). This review aims to provide a comprehensive summary of these separation methods, offering a critical discussion of their strengths and limitations in the analysis of boswellic acids. The knowledge of various separation methods plays a pivotal role in the quality control of herbal dietary supplements and the monitoring of the metabolism and pharmacokinetics of their constituents. The approaches based on metabolomics and network pharmacology represent new ways of fingerprinting secondary metabolites in resin increasing the comprehensiveness of the output of these methods resulting in safer dietary supplements.
Crit Rev Anal Chem. 2024 Mar;():1-27.
PMID: 38462842 [PubMed - as supplied by publisher]
|
2. |
Rapid Detection of Adulteration in Extracts with Citric Acid by UPLC-HRMS and H NMR.
Püski P, Körmöczi T, Berkecz R, Barta A, Bajtel Á, Kiss T
ole-gum-resin extracts (BSEs) are commonly used as food supplements, especially in osteoarthritis management. The quality standard is established by determining 11-keto-β-boswellic acid (KBA) and acetyl-11-keto-boswellic acid (AKBA) content using high-performance liquid chromatography (HPLC) or assessing the total boswellic acid (TBA) content by titrimetry. The limited geographical distribution of species and increasing industrial demand could increase the risk of adulteration in -containing products. In this study, 14 BSEs from commercial sources, used in food supplements, were analyzed in comparison with a USP Reference Standard extract. The KBA and AKBA content was determined by HPLC, whereas the TBA content was determined by titration. Targeted UHPLC-high-resolution mass spectrometry (HRMS) was applied to identify the carboxylic acid content in the samples. The H NMR spectra of extracts were also analyzed. Only two products met the criteria for KBA and AKBA content. Although, the TBA content complied with the expected amount, 10 extracts contained citric acid levels of 6-11% even though citric acid is not a cha-racteristic component of BSEs. Our results suggest undeclared addition of citric acid to comply with declared contents of TBA when using titration methods. Incorporation of citric acid to industrial samples - in order to alter the outcomes of the titration analysis - was demonstrated for the first time.
J Diet Suppl. 2024 Jan;():1-16.
PMID: 38165273 [PubMed - as supplied by publisher]
|
3. |
Simultaneous identification and quantification of pentacyclic triterpenoids and phenolic compounds from the leaves of Boswellia serrata using LC-MS/MS tandem mass spectrometry.
Trivedi MK, Branton A, Trivedi D, Sharma T, Mondal S, Jana S
Boswellia serrata (B. serrata) is an important medicinal plant widely used as dietary supplements to provide a support for osteoarthritic and inflammatory diseases. The occurrence of triterpenes in leaves of B. serrata is very little or none. Therefore, the qualitative and quantitative determination of phytoconstituents (triterpenes and phenolics) present in the leaves of B. serrata is very much needed. The aim of this study was to develop an easy, rapid, efficient and simultaneous liquid chromatography-mass spectrometry (LC-MS/MS) method for the identification and quantification of the compounds present in the leaves extract of B. serrata. The purification of ethyl acetate extracts of B. serrata was performed by solid phase extraction method, followed by HPLC-ESI-MS/MS analysis. Chromatographic parameters of the analytical method included negative electrospray ionization (ESI) with a flow of 0.5 mL/min in gradient mode consisting of acetonitrile (A) and water (B) containing 0.1% formic acid, at 20 °C. Total 19 compounds (13 triterpenes and 6 phenolic compounds) were separated, and simultaneously quantified using a validated LC-MS/MS method with high accuracy and sensitivity. Good linearity was obtained with r > 0.973 in the calibration range. The overall recoveries were in a range between 95.78 and 100.2% with relative standard deviations (RSD) below 5% for the entire procedure of matrix spiking experiments. Overall, there was no ion suppression from the matrix. The quantification data showed that the total amount of triterpenes and phenolic compounds in the leaves of B. serrata ethyl acetate extract samples ranged from 14.54 to 102.14 mg/g and 2.14 to 93.12 mg/g of dry extract, respectively. This work provides, for the first time, a chromatographic fingerprinting analysis on the leaves of B. serrata. A rapid, efficient, and simultaneous liquid chromatography-mass spectrometry (LC-MS/MS) method was developed and used for the both identification and quantification of triterpenes and phenolic compounds in the leaves extracts of B. serrata. The method established in this work can be used as quality-control method for other market formulations or dietary supplements containing leaf extract of B. serrata.
Anal Sci. 2023 Oct;39(10):1741-1756.
PMID: 37386278 [PubMed - indexed for MEDLINE]
|
4. |
A Simple HPTLC Approach of Quantification of Serratol and Tirucallic Acid with Boswellic Acids in Boswellia serrata by Validated Densitometric Method with MS/MS Characterization.
Mukadam S, Ghule C, Girme A, Shinde VM, Hingorani L, Mahadik KR
This study was planned to develop a simple high-performance thin-layer chromatography method for qualitative and quantitative estimation of 3-acetyl-11-keto-β-boswellic acid (AKBBA), β-boswellic acid (BBA), 3-oxo-tirucallic acid (TCA) and serratol (SRT) with HPTLC-ESI-MS/MS for characterization in Boswellia serrata Roxb. oleo gum resin extract. The method was developed with hexane-ethyl acetate-toluene-chloroform-formic acid as mobile phase. RF values observed for AKBBA, BBA, TCA and SRT were 0.42, 0.39, 0.53 and 0.72, respectively. The method was validated according to International Council for Harmonisation guidelines. The concentration range for linearity was 100-500 ng/band for AKBBA and 200-700 ng/band for the other three markers with r2 > 0.99. The method resulted in good recoveries as 101.56, 100.68, 98.64 and 103.26%. The limit of detection was noticed as 25 , 37, 54 and 38 ng/band, with a limit of quantification as 76, 114, 116 and 115 ng/band, for AKBBA, BBA, TCA and SRT, respectively. The four markers were identified and confirmed in B. serrata extract using TLC-MS by indirect profiling by LC-ESI-MS/MS and were identified as terpenoids, TCA and cembranoids: AKBBA (mass/charge (m/z) = 513.00), BBA (m/z = 455.40), 3-oxo-tirucallic acid (m/z = 455.70) and SRT (m/z = 291.25), respectively.
J Chromatogr Sci. 2023 Dec;61(10):953-962.
PMID: 36892162 [PubMed - indexed for MEDLINE]
|
5. |
Comparison of Volatile Constituents Present in Commercial and Lab-Distilled Frankincense () Essential Oils for Authentication.
Ojha PK, Poudel DK, Rokaya A, Satyal R, Setzer WN, Satyal P
A comparative analysis of the chemical constituents present in twenty-one commercial and two lab-distilled frankincense () essential oils was carried out using gas chromatography-mass spectrometry (GC-MS) and chiral gas chromatography-mass spectrometry (CGC-MS) for authentication. Out of the twenty-one commercial samples, six were adulterated with synthetic limonene, three were contaminated with synthetic octyl acetate, three were adulterated with castor oil, and two samples each were contaminated with frankincense resin and species, respectively, and one was contaminated with the species. Additionally, one sample was contaminated with phthalates as well as a cheap essential oil with similar compositions. Furthermore, one sample was adulterated with copaiba resin and frankincense resin in combination with synthetic octyl acetate. Additionally, one was contaminated with species, which was further adulterated with castor oil and frankincense resin. To the best of our knowledge, this is the first report to compare the enantiomeric distribution of chiral terpenoids present in commercial frankincense essential oil with lab-distilled frankincense oil for authentication. The CGC-MS analysis showed the presence of a total of eight chiral terpenoids in lab-distilled frankincense essential oils, which can be used as chemical fingerprints for the authentication of frankincense essential oil.
Plants (Basel). 2022 Aug;11(16):.
PMID: 36015437 [PubMed - as supplied by publisher]
|
6. |
Retention index based approach for simulation of results and application for validation of compound identification in comprehensive two-dimensional gas chromatography.
Kakanopas P, Janta P, Vimolmangkang S, Hermatasia F, Kulsing C
In this work, first and second dimensional retention index (I and I) based calculation approach is established to simulate peak retention times (t and t) of samples for the given sets of volatile compounds in comprehensive two-dimensional gas chromatography-mass spectrometry (GC×GC-MS). For the result without t and t data of alkane references (t and t), the following steps were applied: (1) curve fitting based on van den Dool and Kratz relationship in order to simulate t using a training set of volatile compounds in a sample with their experimental t data, and (2) simulation of t at different t to construct their isovolatility curves based on a nonlinear equation with p-p parameters and a constant (within the ranges of -0.0052 to 0.0049, -0.6181 to -0.0230, -26.4775 to -0.2698, 0.0050 to 9.6259, -7.2976 to -3.9524 and 0.9157 to 4.0779, respectively). These parameters were obtained by performing curve fitting according to the experimental t data of the same training set with the least square values ranging from 4.58×10 to 32.55. Simulation of t and t of target analytes (t and t) with known I and I were performed using t and the simulated isovolatility curves. All the calculations and curve fittings were carried out by using Solver in Microsoft Excel. The approach was applied to simulate results for 542 compounds in several samples including analysis of saffron (Crocus sativas L.), Boswellia papyrifera, acacia honey and incense powder/smoke, perfume and cannabis either reported from literature or from the experiments in this work using different experimental approaches. These were compared with the experimental data showing correlation with the R ranges of 0.98-1.00 and 0.80-0.97 for t and t, respectively. This approach was then applied to propose 6 compounds which may be incorrectly identified based on the differences of >2 times of the standard deviations between t and the experimental t in both residue and leave-one-out analyses.
J Chromatogr A. 2022 Aug;1679():463394.
PMID: 35970049 [PubMed - indexed for MEDLINE]
|
7. |
Analysis of boswellic acids in dietary supplements containing Indian frankincense (Boswellia serrata) by Supercritical Fluid Chromatography.
Zwerger M, Ganzera M
Boswellic acids, a class of triterpenes, are the bioactive constituents in Indian frankincense, an herbal drug with pronounced anti-inflammatory activity. In this study their separation and quantification in B. serrata extracts is reported for the first time by using Supercritical Fluid Chromatography. Under optimized conditions, i.e. a Viridis HSS C18 SB column and carbon dioxide, methanol, acetonitrile and ammonium hydroxide as mobile phase, six boswellic acids could be separated in under 6 min. The assay fulfilled all validation criteria with coefficients of determination higher than 0.999, a wide linear range (30-1000 μg/mL), recovery rates from 97.1-103.0 %, excellent precision, and detection limits typical for SFC with UV-detection (≤ 5.5 μg/mL). The method could easily be hyphenated to mass spectrometry, which was helpful to tentatively assign further compounds (mainly derivatives of tirucallic acid) and to increase the assay's sensitivity. Its practical applicability was confirmed by analyzing several commercial products, which mainly contained β-boswellic acid as dominant triterpene, yet in extremely variable amounts ranging from 0.9 to 16.9 %.
J Pharm Biomed Anal. 2021 Jul;201():114106.
PMID: 33962180 [PubMed - indexed for MEDLINE]
|
8. |
Rapid Identification of Commercial Frankincense Products by MALDITOF Mass Spectrometry.
Lai SC, You RI, Chen TT, Chang Y, Liu CZ, Chen HP, Wu C
BACKGROUND: Frankincense is a resin secreted by the Boswellia tree. It is used in perfumery, aromatherapy, skincare, and traditional Chinese medicine. However, all Boswellia species are under threat owing to habitat loss and overexploitation. As a result, the market is getting flooded with counterfeit frankincense products.
OBJECTIVE: This study aims to establish a high-throughput method to screen and identify the authenticity of commercial frankincense products. We report, for the first time, a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based method for rapid and high-throughput screening of frankincense samples.
METHODS: MALDI-TOF MS, HPLC, thin-layer chromatography (TLC), and in vitro antiinflammatory activity assay were used to examine the frankincense samples.
RESULTS: Well-resolved peaks of frankincense triterpenoids in the spectra were observed in the crude extract of commercial samples, including α-boswellic acids (αBAs), β-boswellic acids (βBAs), 11-keto-β-boswellic acids (KBAs), acetyl-11-keto-β-boswellic acids (AKBAs), and their esters. These compounds can be used as indicators for determining the authenticity of frankincense.
CONCLUSION: Unlike LC-MS, which is a time-consuming and expensive method, and TLC, which requires a reference sample, our inexpensive, rapid high-throughput identification method based on MALDI-TOF MS is ideal for large-scale screening of frankincense samples sold in the market.
Comb Chem High Throughput Screen. 2022;25(5):895-905.
PMID: 33645476 [PubMed - indexed for MEDLINE]
|
9. |
Development and validation of a sensitive UHPLC-MS/MS method for the measurement of β-elemonic acid in rat plasma and tissues and its application to pharmacokinetics and tissue distribution study.
Zhang Y, Huo XX, Cheng LY, Chen MY, Song L, Yu YL, Zhou K
β-Elemonic acid is one of the main active ingredients isolated from Boswellia carterii Birdw. which has been reported to exhibit potential anti-inflammatory and anti-cancer activities. There is few information about pharmacokinetics and tissue distribution of β-elemonic acid by now. In this study, an ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-MS/MS) method has been developed and validated to determine β-elemonic acid in rat plasma and various tissues after intragastric administration. Oleanolic acid was chosen as an internal standard (IS) and the plasma/tissue samples were pretreated with one-step liquid-liquid extraction. Chromatographic separation was accomplished on Eclipse Plus C18 analytical column (2.1 × 50 mm, 1.8 μm) utilizing a gradient mobile phase system consisting of water (with 0.1% ammonia-solution) and acetonitrile. β-Elemonic acid and IS were detected and quantified using negative electrospray ionization in multiple reaction monitoring (MRM) mode with transitions of m/z 453.3 → 423.5 for β-elemonic acid and m/z 455.3 → 407.6 for IS. β-Elemonic acid showed good linearity over the investigated concentration range (r > 0.9934) in rat plasma and tissue sample. The method was successfully applied for determination of β-elemonic acid in bio-samples. A bimodal phenomenon appeared in the plasma concentration-time curve of the β-elemonic acid. The highest tissue concentrations were found in the intestine including jejunum, ileum and colon.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Mar;1167():122566.
PMID: 33578281 [PubMed - indexed for MEDLINE]
|
10. |
Extraction of 11-Keto-β -Boswellic Acid from Indian Olibanum by Contemporary Extraction Modes: Optimization and Validation of HPTLC.
Abidin L, Singla P, Kausar H, Mujeeb M, Rahman A, Aqil M, Najmi AK
BACKGROUND: Boswellic acids are the main constituents of Boswellia serrata gum. These comprise of four pentacyclic triterpenes, 11-keto-β-boswellic acid (KBA) being one of them.
OBJECTIVES: Comparing the extraction efficiency of KBA through microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) followed by optimizing the extraction process using response surface methodology (RSM) and validation of HPTLC.
METHODS: UAE and MAE of KBA were carried out employing methanol as an extracting solvent. To figure out the better mode of extraction, single factorial experiments were conducted for further optimization. Design expert software was used for optimization purposes where solvent to drug ratio, extraction temperature and extraction time were taken as input variables. Quantification of KBA in each extract was done through High-Performance Thin Layer Chromatography (HPTLC), and the method was validated as per International Council for Harmonization (ICH) guidelines.
RESULTS: UAE stood out to be a better mode of extraction for KBA. Solvent to drug ratio of 20.42 mL/gram, extraction temperature of 44.01°C and extraction time of 11.54 minutes were established as optimum conditions, which yielded 8.44%w/w of KBA. Regarding HPTLC, the Rf value of KBA was measured, and the correlation coefficient was calculated from the standard curve. Accuracy, precision and recovery were found within limits.
CONCLUSION: From this study, it was concluded that a non-thermal method is a better choice of extraction for KBA. All the input variables significantly affected KBA content, which was confirmed by model fitting. Moreover, the HPTLC method was developed for the quantification of KBA, which was found to be accurate, reliable and highly sensitive.
Comb Chem High Throughput Screen. 2021;24(8):1309-1321.
PMID: 32928084 [PubMed - indexed for MEDLINE]
|
11. |
A Validated LC-MS/MS Method for Simultaneous Determination of 3-O-Acetyl-11-Keto-β-Boswellic Acid (AKBA) and its Active Metabolite Acetyl-11-Hydroxy-β-Boswellic Acid (Ac-11-OH-BA) in Rat Plasma: Application to a Pharmacokinetic Study.
Sharma T, Jana S
The aim of this study was to develop and validate a new, rapid, sensitive, selective and reliable liquid chromatography-tandem mass spectrometry method for simultaneous determination of 3-O-Acetyl-11-keto-β-boswellic acid (AKBA) and its active metabolite 3-O-Acetyl-11-hydroxy-β-boswellic acid (Ac-11-hydroxy-BA) in rat plasma. Both analytes (AKBA and Ac-11-hydroxy-BA) and the internal standard (IS, ursolic acid) were extracted from 100 μL of rat plasma by protein precipitation. Chromatographic separation was achieved on PRP-H1 RP-C18 column (75 mm × 2 mm, 1.6 μm) using acetonitrile-water (95.5 v/v) as the mobile phase. Mass detection was conducted by electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. A linear dynamic range of 1-1,000 ng/mL for both AKBA and Ac-11-hydroxy-BA was established with mean correlation coefficient (r (1)) of 0.999. Intra- and inter-day precision (% CV) of analysis were found in the range of 1.9-7.4%. The accuracy determined for these analytes ranged from 92.4 to 107.2%. The extraction recoveries for both analytes ranged from 92.6 to 97.3% for spiked plasma samples and were consistent. The % change in stability samples compared to nominal concentration ranged from 0.4 to 4.2%. This method was successfully tested to a pharmacokinetic (PK) study for estimation of AKBA and acetyl-11-hydroxy-BA in rat plasma following oral administration of AKBA. This method has been validated with the advantage of shorter run time that can be used for high-throughput analysis and has been successfully applied to the pharmacokinetic study of AKBA in rats.
J Chromatogr Sci. 2020 Jun;58(6):485-493.
PMID: 32134105 [PubMed - indexed for MEDLINE]
|
12. |
Exploiting the versatility of vacuum-assisted headspace solid-phase microextraction in combination with the selectivity of ionic liquid-based GC stationary phases to discriminate Boswellia spp. resins through their volatile and semivolatile fractions.
Capetti F, Rubiolo P, Bicchi C, Marengo A, Sgorbini B, Cagliero C
The frankincense resins, secreted from Boswellia species, are an uncommon example of a natural raw material where every class of terpenoids is present in similar proportions. Diterpenoids (serratol, incensole, and incensole acetate) are used to discriminate samples from different species and origins. Headspace solid-phase microextraction has been used for frankincense analysis, although it requires long sampling time for medium- to low-volatility markers; headspace solid-phase microextraction under vacuum can overcome this limit. Gas chromatography is used for analysis but the separation of incensole and serratol needs polar stationary phases. In this study, we develop a method to discriminate frankincenses based on vacuum-assisted headspace solid-phase microextraction combined with fast gas chromatography-mass spectrometry with ionic liquid-based stationary phases. The optimized conditions for solid samples were: air evacuation below 0°C, 15 min of incubation time, and 15 min of extraction time. Losses of volatiles due to vial air-evacuation in the presence of the sample were minimized by sample amount above 100 mg and low sample temperature. Fast gas chromatography provides the baseline separation of all markers in 20 min. By applying vacuum sampling and fast gas chromatography, the total analysis was reduced to 50 min compared to 120 min (60 min sampling plus 60 min analysis) as previously reported. The method was successfully applied to commercial frankincense samples.
J Sep Sci. 2020 May;43(9-10):1879-1889.
PMID: 32072762 [PubMed - indexed for MEDLINE]
|
13. |
Estimation of boswellic acids in herbal formulations containing extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS.
Katragunta K, Siva B, Kondepudi N, Vadaparthi PRR, Rama Rao N, Tiwari AK, Suresh Babu K
is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties. A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds, i.e., keto boswellic acid (), 3-O-Acetyl 11-keto β-boswellic acid (), ɑ-Boswellic acid (), β-Boswellic acid (), 3-O-Acetyl-ɑ-boswellic acid () and 3-O-Acetyl-β-boswellic acid () in commercial herbal products containing as an ingredient. Combining UPLC with Q-Tof-MS/MS makes the better identification of secondary metabolites and adulterants in the herbal formulations containing in rapid time using fragmentation approach than the traditional approaches. In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines. Furthermore, minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in oleo-gum-resin extract were identified.
J Pharm Anal. 2019 Dec;9(6):414-422.
PMID: 31890341 [PubMed - as supplied by publisher]
|
14. |
Biophysical properties and finger print of Sp. .
Alotaibi SH
For the first time a finger print analysis via high-performance thin layer chromatography (HPTLC) of Sp. was accomplished. A preliminary investigation of the Sp. displayed the presence of chemical constituents that could be involved in the production of innovative pharmaceuticals for an array of antiviral, anticancer, and antibacterial uses. Moreover, the finger print analysis would deem useful for establishing HPTLC standardization for natural and herbal photochemical constituents.
Saudi J Biol Sci. 2019 Nov;26(7):1450-1457.
PMID: 31762608 [PubMed - as supplied by publisher]
|
15. |
Corrigendum to "Analytical investigations on Boswellia occulta essential oils" [Phytochemistry 164 (2019) 78-85].
Ayubova M, Brevard H, Baldovini N
Phytochemistry. 2019 Sep;165():112053.
PMID: 31253423 [PubMed - as supplied by publisher]
|
16. |
Analytical investigations on Boswellia occulta essential oils.
Ayubova M, Guelleh ZO, Guelleh MO, Brévard H, Baldovini N
Three samples of Boswellia occulta gum resin (Grades I, II and III) were analyzed by GC-MS and GC-FID. Fifty constituents could be identified, and several of them were isolated by flash chromatography and characterized by NMR. The combinatorial synthesis of homologous series of reference constituents permitted the unambiguous identification of five 1-methoxyalkanes and one 1-methoxyalkene. In addition, we measured the C content of one sample of essential oil and of a methoxyalkanes rich fraction and demonstrated that the origin of these materials is totally natural.
Phytochemistry. 2019 Aug;164():78-85.
PMID: 31102998 [PubMed - indexed for MEDLINE]
|
17. |
Compositional analysis of the essential oil of Boswellia dalzielii frankincense from West Africa reveals two major chemotypes.
DeCarlo A, Johnson S, Okeke-Agulu KI, Dosoky NS, Wax SJ, Owolabi MS, Setzer WN
Frankincense, an oleoresin produced by Boswellia species, has historical medicinal and religious significance, and is today used extensively for its essential oil. Boswellia dalzielii, a species found in West Africa, is one of the few frankincense species for which there is no information on the oleoresin essential oil. In order to correct this deficiency, the chemical compositions of the essential oil hydrodistilled from 21 samples of oleoresin taken directly from B. dalzielii trees in northern Nigeria, were analyzed by gas chromatography - mass spectrometry as well as chiral gas chromatography. In addition, a hierarchical cluster analysis was performed on the essential oil compositions from the 21 oleoresin samples from northern Nigeria as well as two samples from Ghana. The essential oil fractions obtained by hydrodistillation of B. dalzielii oleoresins were dominated by α-pinene (21.7-76.6%), followed by α-thujene (2.0-17.6%), myrcene (up to 35.2%), p-cymene (0.3-15.6%), and limonene (1.1-32.9%). The levorotatory enantiomers predominated for the monoterpenes with 98.1 ± 1.5% (-)-α-thujene, 99.2 ± 0.5% (-)-α-pinene, and 96.8 ± 1.4% (-)-β-pinene. Limonene showed the largest variation in enantiomeric distribution [67.3 ± 12.1% (-)-limonene]. The cluster analysis revealed two major chemotypes, one dominated by α-pinene and one much rarer chemotype rich in myrcene.
Phytochemistry. 2019 Aug;164():24-32.
PMID: 31071599 [PubMed - indexed for MEDLINE]
|
18. |
Optimal Processing Conditions of Birdw. Using Response Surface Methodology.
Yoon JH, Kim JH, Ham SS, Gang BY, Lee SH, Choi G, Kim YS, Lee G, Ju YS
BACKGROUND: Bridw. is being widely used for its anti-inflammatory properties, as well as for wound healing, antimicrobial, and immunomodulatory properties, and boswellic acids (BAs) are considered to be the main active constituents.
OBJECTIVES: To investigate optimal conditions of stir-baking process for the resin of with vinegar of using response surface methodology (RSM).
MATERIALS AND METHODS: The concentration of acetic acid, heating temperature, and heating time were set as influential factors, and the yields of chemical compounds were the response values which were optimally designed by a Box-Behnken design. The amounts of 11-keto-β-boswellic acid (KBA) and α-boswellic acid (αBA) in resin were quantified using high-performance liquid chromatography analysis.
RESULTS: Maximum amounts of KBA and αBA in resin were obtained using 6% acetic acid for 10 min at 90°C in preliminary test. Two factor interactions, such as acetic acid concentration-heating temperature and heating temperature-heating time, were significantly observed by multiple regression analysis. Optimal processing conditions from RSM were 5.83% for acetic acid concentration, 9.56 min for heating time, and 89.87°C for heating temperature. Under the modified conditions, the experimental value of the response was 11.25 mg/g, which was similar to the predicted value.
CONCLUSIONS: The results suggest that the optimal conditions for the stir-baking process of resin were determined by RSM, which was reliable and applicable to practical processing of herbal medicine.
SUMMARY: The resin of was macerated in aqueous acetic acid and heated using an oven for stir baking processThe interaction between heating temperature and heating time was the most significantOptimal conditions for processing resin were determined as 5.83% acetic acid, 9.56 min for heating time, and 89.87°C for heating temperature. BAs: Boswellic acids; KBA: 11 keto β boswellic acid; αBA: α boswellic acid; BBD: Box-Behnken design; RSM: Response surface method; HPLC: High performance liquid chromatography; LOD: Limits of determination; LOQ: Limits of quantification; RSD: Relative standard deviation; ANOVA: Analysis of variance.
Pharmacogn Mag. 2018;14(54):235-241.
PMID: 29720838 [PubMed - as supplied by publisher]
|
19. |
Comprehensive 2D gas chromatography-time-of-flight mass spectrometry with 2D retention indices for analysis of volatile compounds in frankincense (Boswellia papyrifera).
Jiang M, Kulsing C, Marriott PJ
Frankincense gum resin secreted from Boswellia papyrifera was analysed by comprehensive 2D gas chromatography hyphenated with accurate mass time-of-flight mass spectrometry (GC×GC-accTOFMS). Direct multiple injection experiments with stepwise isothermal temperature programming were then performed to construct isovolatility curves for reference alkane series in GC×GC. This provides access to calculation of second dimensional retention indices (I). More than 500 peaks were detected and 220 compounds mainly comprising monoterpenes, sesquiterpenes, diterpenes and oxygenated forms of these compounds were identified according to their I, I and accurate mass data. The study demonstrates the capability of GC×GC-accTOFMS with retention data on two separate column phases, as an approach for improved component identification. A greater number of identified and/or tentatively identified terpenoids in this traditional Chinese medicine allow for a more comprehensive coverage of the volatile composition of frankincense.
Anal Bioanal Chem. 2018 May;410(13):3185-3196.
PMID: 29582122 [PubMed - indexed for MEDLINE]
|
20. |
Quantification of AKBA in Boswellia sacra Using NIRS Coupled with PLSR as an Alternative Method and Cross-Validation by HPLC.
Rehman NU, Ali L, Al-Harrasi A, Mabood F, Al-Broumi M, Khan AL, Hussain H, Hussain J, Csuk R
INTRODUCTION: 3-O-Acetyl-11-keto-β-boswellic acid (AKBA), one of the pentacyclic triterpenoids, is the main biologically active constituent in the resin of Boswellia sacra and has received significant pharmacological interest in recent years.
OBJECTIVE: It was aimed to develop a robust method to quantify the AKBA content in methanolic extracts of different parts of B. sacra plants and in various fractions of its resin exudates through near-infrared spectroscopy (NIRS) coupled with partial least squares regression (PLSR).
MATERIAL AND METHODS: The near-infrared (NIR) spectra were used to measure the AKBA standards and B. sacra samples at a wavelength range between 700 and 2500 nm in absorption mode. A PLSR model was built from the obtained spectral data using 70% of the AKBA working standard solutions (training set), ranging from 0.1 ppm to 100 ppm. The final PLSR showed a R value of 99% with a root mean square error of cross-validation (RMSECV) value of 0.39% and a R value of 99%.
RESULTS: The results showed that a 50% CHCl /n-hexane sub-fraction has the highest concentration of AKBA (14.8%), followed by 55% CHCl /n-hexane (13.6%), and 40% CHCl /n-hexane (6.1%).
CONCLUSION: As the results achieved with the proposed NIRS methodology are in close agreement to the results of AKBA analysis using HPLC, we suggest that our proposed NIRS method is a fast alternative and non-destructive method for the analysis of AKBA in different samples of B. sacra. Copyright © 2017 John Wiley & Sons, Ltd.
Phytochem Anal. 2018 Mar;29(2):137-143.
PMID: 28881407 [PubMed - indexed for MEDLINE]
|
21. |
Extraction and purification of five terpenoids from olibanum by ultrahigh pressure technique and high-speed countercurrent chromatography.
Yu J, Zhao H, Wang D, Song X, Zhao L, Wang X
Five terpenoids, including two new ones, 3,7-dioxo-tirucalla-8,24-dien-21-oic acid (2) and 3α-acetoxyl-7-oxo-tirucalla-8,24-dien-21-oic acid (3), and three known ones, boscartol A (1), 11-keto-β-boswellic acid (4), and acetyl-11-keto-boswellic acid (5), have been extracted by the ultrapressure extraction and purified by pH-zone-refining countercurrent chromatography and high-speed countercurrent chromatography from olibanum. For ultrapressure extraction, the optimal condition including 200 MPa of extraction pressure, ethyl acetate of extraction solvent, 1:20 (g/mL) of solid/liquid ratio, and 2 min of extraction time were obtained. For the separation, from 1.5 g of the terpenoid extract, 220.1 mg of 4, 255.5 mg of 5, and 212.3 mg of the mixture of 1, 2, and 3 were obtained by pH-zone-refining countercurrent chromatography under the solvent system of chloroform/ethyl acetate/methanol/water (3:1:3:2, v/v/v/v) with aqueous ammonia and trifluoroacetic acid as retention and eluter agents. The enriched mixture (210 mg) was further separated by conventional high-speed countercurrent chromatography with petroleum ether/ethyl acetate/methanol/water (1:0.8:1.1:0.6, v/v/v/v), yielding 30.1 mg of 1, 35.5 mg of 2, 12.3 mg of 3. The structures of these five terpenoids were elucidated by extensive spectroscopic methods.
J Sep Sci. 2017 Jul;40(13):2732-2740.
PMID: 28544633 [PubMed - indexed for MEDLINE]
|
22. |
Application of NIRS coupled with PLS regression as a rapid, non-destructive alternative method for quantification of KBA in Boswellia sacra.
Al-Harrasi A, Rehman NU, Mabood F, Albroumi M, Ali L, Hussain J, Hussain H, Csuk R, Khan AL, Alam T, Alameri S
In the present study, for the first time, NIR spectroscopy coupled with PLS regression as a rapid and alternative method was developed to quantify the amount of Keto-β-Boswellic Acid (KBA) in different plant parts of Boswellia sacra and the resin exudates of the trunk. NIR spectroscopy was used for the measurement of KBA standards and B. sacra samples in absorption mode in the wavelength range from 700-2500nm. PLS regression model was built from the obtained spectral data using 70% of KBA standards (training set) in the range from 0.1ppm to 100ppm. The PLS regression model obtained was having R-square value of 98% with 0.99 corelationship value and having good prediction with RMSEP value 3.2 and correlation of 0.99. It was then used to quantify the amount of KBA in the samples of B. sacra. The results indicated that the MeOH extract of resin has the highest concentration of KBA (0.6%) followed by essential oil (0.1%). However, no KBA was found in the aqueous extract. The MeOH extract of the resin was subjected to column chromatography to get various sub-fractions at different polarity of organic solvents. The sub-fraction at 4% MeOH/CHCl (4.1% of KBA) was found to contain the highest percentage of KBA followed by another sub-fraction at 2% MeOH/CHCl (2.2% of KBA). The present results also indicated that KBA is only present in the gum-resin of the trunk and not in all parts of the plant. These results were further confirmed through HPLC analysis and therefore it is concluded that NIRS coupled with PLS regression is a rapid and alternate method for quantification of KBA in Boswellia sacra. It is non-destructive, rapid, sensitive and uses simple methods of sample preparation.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Sep;184():277-285.
PMID: 28525862 [PubMed - indexed for MEDLINE]
|
23. |
Quantitative Determination of 3-O-Acetyl-11-Keto-βBoswellic Acid (AKBA) and Other Boswellic Acids in Boswellia sacra Flueck (syn. B. carteri Birdw) and Boswellia serrata Roxb.
Mannino G, Occhipinti A, Maffei ME
Boswellia serrata and Boswellia sacra (syn. B. carteri) are important medicinal plants widely used for their content of bioactive lipophilic triterpenes. The qualitative and quantitative determination of boswellic acids (BAs) is important for their use in dietary supplements aimed to provide a support for osteoarthritic and inflammatory diseases. We used High Performance Liquid Chromatography (HPLC)-Diode Array Detector (DAD) coupled to ElectroSpray Ionization and tandem Mass Spectrometry (ESI-MS/MS) for the qualitative and quantitative determination of BAs extracted from the gum resins of B. sacra and B. serrata. Limit of detection (LOD), limit of quantification (LOQ), and Matrix Effect were assessed in order to validate quantitative data. Here we show that the BAs quantitative determination was 491.20 g·kg-1 d. wt (49%) in B. sacra and 295.25 g·kg-1 d. wt (30%) in B. serrata. Lower percentages of BAs content were obtained when BAs were expressed on the gum resin weight (29% and 16% for B. sacra and B. serrata, respectively). The content of Acetyl-11-Keto-β-Boswellic Acid (AKBA) was higher in B. sacra (70.81 g·kg-1 d. wt; 7%) than in B. serrata (7.35 g·kg-1 d. wt; 0.7%). Our results show that any claim of BAs content in either B. sacra or B. serrata gum resins equal to or higher than 70% or AKBA contents of 30% are simply unrealistic or based on a wrong quantitative determination.
Molecules. 2016 Oct;21(10):.
PMID: 27782055 [PubMed - indexed for MEDLINE]
|
24. |
Simultaneous quantification of triterpenoic acids by high performance liquid chromatography method in the extracts of gum resin of Boswellia serrata obtained by different extraction techniques.
Sharma N, Bhardwaj V, Singh S, Ali SA, Gupta DK, Paul S, Satti NK, Chandra S, Verma MK
BACKGROUND: Boswellia serrata, also known as Indian frankincense is a commercially important medicinal plant which has been used for hundreds of years as an Ayurvedic medicine for the attempted treatment of arthritis. It contains naturally occurring triterpenoic acids, called as boswellic acids (BA's).
RESULTS: A highly reproducible High performance liquid chromatography-ultraviolet diode array detection (HPLC-UV-DAD) method was developed for the simultaneous determination and quantitative analysis of eight major triterpenoic acids in Boswellia serrata gum resin obtained by different extraction techniques. All the calibration curves exhibited good linear regression (R(2) > 0.997) within the test ranges. The established method showed good precision and overall recoveries of the boswellic acids.
CONCLUSIONS: The eight triterpenoic acids coded as BS-1 (11-keto-beta-boswellic acid), BS-2 (3-O-acetyl-11-keto-beta-boswellic acid), BS-3 (3-keto tirucallic acid), BS-4 (3-O-acetyl-alpha-tirucallic acid), BS-5 (3-O-acetyl-beta-tirucallic acid), BS-6 (alpha-boswellic acid), BS-7 (beta-boswellic acid) and BS-8 (3-O-acetyl-beta-boswellic acid) were isolated from the processed gum resin of Boswellia serrata by column chromatography. The proposed HPLC method is simple, reliable and has been very useful for the qualitative as well as quantitative analysis of boswellic acids in the gum resin of Boswellia serrata. The proposed method allows to quantify boswellic acids in appreciable amounts by HPLC-UV (DAD) method in the extracts and the available marketed formulations.Graphical abstractIsolation & separation of eight Triterpenoic acids from Boswellia serrata.
Chem Cent J. 2016;10():49.
PMID: 27493682 [PubMed - as supplied by publisher]
|
25. |
Development of a gas chromatography-mass spectrometry method to monitor in a single run, mono- to triterpenoid compounds distribution in resinous plant materials.
Jemmali Z, Chartier A, Elfakir C
A new procedure based on gas chromatography coupled to mass spectrometry (GC-MS) was developed for the simultaneous determination of mono- to triterpenoid compounds in resinous materials. Given the difference of volatility and polarity of the studied compounds some critical steps in this methodology had to be identified and investigated. The recovery of volatile compounds after sample extraction was studied. A recovery range from 30% to 100% from the more volatile monoterpene to the least one was observed. Then the mandatory derivatization step for the analysis of pentacyclic triterpenes bearing hydroxyl and carboxyl groups was optimized. Results showed that derivatization using N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS) in pyridine (22:13:65 v/v/v) for 2h at 30 °C was the most efficient method of derivatizing all the hydroxyl and carboxylic acid groups contained in the triterpene structures. After choosing the best injection parameters for these compounds, the selectivity of the GC column towards the separation of these terpenoids was investigated using statistical tools (principal component analysis and desirability functions). A separation with a good resolution was achieved on an HP-5ms column using a programmed temperature vaporizing injector (PTV). The method was pre-validated in terms of detection limits (LOD from 100 μg L(-1) to 200 μg L(-1) depending on the compound), linearity and repeatability using seven compounds representative of mono- and triterpenoid classes. An exhaustive characterization of various types of resins (di-, triterpenic and oleo-gum resins) was achieved.
J Chromatogr A. 2016 Apr;1443():241-53.
PMID: 27018190 [PubMed - indexed for MEDLINE]
|
26. |
Frankincense Revisited, Part II: Volatiles in Rare Boswellia Species and Hybrids.
Niebler J, Eslamieh J, Buettner A
In this second part of the investigation of volatiles and semivolatiles in Boswellia gum resins, an additional five less common species were analyzed by (SPME-)GC/MS, namely B. ameero, B. elongata, B. neglecta, B. popoviana, and B. rivae. Moreover, the results of hybridization experiments are reported in combination with the volatile composition of their gum resins. Our study shows that B. sacra benefits from an intraspecific cross-pollination, as the resulting hybrid B. sacra var. supersacra has a far higher seed germination rate and viability.
Chem Biodivers. 2016 May;13(5):630-43.
PMID: 27012302 [PubMed - indexed for MEDLINE]
|
27. |
Fragrant Sesquiterpene Ketones as Trace Constituents in Frankincense Volatile Oil of Boswellia sacra.
Niebler J, Zhuravlova K, Minceva M, Buettner A
In a previous study, two highly potent yet unidentified odorants were detected that were present at trace levels in the volatile fraction of Boswellia sacra gum resin. These two compounds were isolated semipreparatively from the volatile oil by a sensory-guided fractionation process involving microscale bulb-to-bulb distillation, countercurrent chromatography, and preparative gas chromatography. In this manner, the two oxygenated sesquiterpenes could be identified as rotundone (1) and mustakone (2). Compound 2 is described for the first time as a potent odorant with a very low odor threshold.
J Nat Prod. 2016 Apr;79(4):1160-4.
PMID: 27010489 [PubMed - indexed for MEDLINE]
|
28. |
Phytochemical Analysis and Anti-cancer Investigation of Boswellia serrata Bioactive Constituents In Vitro.
Ahmed HH, Abd-Rabou AA, Hassan AZ, Kotob SE
Cancer is a major health obstacle around the world, with hepatocellular carcinoma (HCC) and colorectal cancer (CRC) as major causes of morbidity and mortality. Nowadays, there isgrowing interest in the therapeutic use of natural products for HCC and CRC, owing to the anticancer activity of their bioactive constituents. Boswellia serrata oleo gum resin has long been used in Ayurvedic and traditional Chinese medicine to alleviate a variety of health problems such as inflammatory and arthritic diseases. The current study aimed to identify and explore the in vitro anticancer effect of B. Serrata bioactive constituents on HepG2 and HCT 116 cell lines. Phytochemical analysis of volatile oils of B. Serrata oleo gum resin was carried out using gas chromatography- mass spectrometry (GC/MS). Oleo-gum-resin of B. Serrata was then successively extracted with petroleum ether (extract 1) and methanol (extract 2). Gas-liquid chromatography (GLC) analysis of the lipoidal matter was also performed. In addition, a methanol extract of B. Serrata oleo gum resin was phytochemically studied using column chromatography (CC) and thin layer chromatography (TLC) to obtain four fractions (I, II, III and IV). Sephadex columns were used to isolate β-boswellic acid and identification of the pure compound was done using UV, mass spectra, 1H NMR and 13C NMR analysis. Total extracts, fractions and volatile oils of B. Serrata oleo-gum resin were subsequently applied to HCC cells (HepG2 cell line) and CRC cells (HCT 116 cell line) to assess their cytotoxic effects. GLC analysis of the lipoidal matter resulted in identification of tricosane (75.32%) as a major compound with the presence of cholesterol, stigmasterol and β-sitosterol. Twenty two fatty acids were identified of which saturated fatty acids represented 25.6% and unsaturated fatty acids 74.4% of the total saponifiable fraction. GC/MS analysis of three chromatographic fractions (I,II and III) of B. Serrata oleo gum resin revealed the presence of pent-2-ene-1,4-dione, 2-methyl- levulinic acid methyl ester, 3,5- dimethyl- 1- hexane, methyl-1-methylpentadecanoate, 1,1- dimethoxy cyclohexane, 1-methoxy-4-(1-propenyl)benzene and 17a-hydroxy-17a-cyano, preg-4-en-3-one. GC/MS analysis of volatile oils of B. Serrata oleo gum resin revealed the presence of sabinene (19.11%), terpinen-4-ol (14.64%) and terpinyl acetate (13.01%) as major constituents. The anti-cancer effect of two extracts (1 and 2) and four fractions (I, II, III and IV) as well as volatile oils of B. Serrata oleo gum resin on HepG2 and HCT 116 cell lines was investigated using SRB assay. Regarding HepG2 cell line, extracts 1 and 2 elicited the most pronounced cytotoxic activity with IC50 values equal 1.58 and 5.82 μg/mL at 48 h, respectively which were comparable to doxorubicin with an IC50 equal 4.68 μg/mL at 48 h. With respect to HCT 116 cells, extracts 1 and 2 exhibited the most obvious cytotoxic effect; with IC50 values equal 0.12 and 6.59 μg/mL at 48 h, respectively which were comparable to 5-fluorouracil with an IC50 equal 3.43 μg/ mL at 48 h. In conclusion, total extracts, fractions and volatile oils of B. Serrata oleo gum resin proved their usefulness as cytotoxic mediators against HepG2 and HCT 116 cell lines with different potentiality (extracts > fractions > volatile oil). In the two studied cell lines the cytotoxic acivity of each of extract 1 and 2 was comparable to doxorubicin and 5-fluorouracil, respectively. Extensive in vivo research is warranted to explore the precise molecular mechanisms of these bioactive natural products in cytotoxicity against HCC and CRC cells.
Asian Pac J Cancer Prev. 2015;16(16):7179-88.
PMID: 26514509 [PubMed - indexed for MEDLINE]
|
29. |
Combination of quantitative analysis and chemometric analysis for the quality evaluation of three different frankincenses by ultra high performance liquid chromatography and quadrupole time of flight mass spectrometry.
Zhang C, Sun L, Tian RT, Jin HY, Ma SC, Gu BR
Frankincense has gained increasing attention in the pharmaceutical industry because of its pharmacologically active components such as boswellic acids. However, the identity and overall quality evaluation of three different frankincense species in different Pharmacopeias and the literature have less been reported. In this paper, quantitative analysis and chemometric evaluation were established and applied for the quality control of frankincense. Meanwhile, quantitative and chemometric analysis could be conducted under the same analytical conditions. In total 55 samples from four habitats (three species) of frankincense were collected and six boswellic acids were chosen for quantitative analysis. Chemometric analyses such as similarity analysis, hierarchical cluster analysis, and principal component analysis were used to identify frankincense of three species to reveal the correlation between its components and species. In addition, 12 chromatographic peaks have been tentatively identified explored by reference substances and quadrupole time-of-flight mass spectrometry. The results indicated that the total boswellic acid profiles of three species of frankincense are similar and their fingerprints can be used to differentiate between them.
J Sep Sci. 2015 Oct;38(19):3324-30.
PMID: 26228790 [PubMed - indexed for MEDLINE]
|
30. |
Identification of odorants in frankincense (Boswellia sacra Flueck.) by aroma extract dilution analysis and two-dimensional gas chromatography-mass spectrometry/olfactometry.
Niebler J, Buettner A
Frankincense has been known, traded and used throughout the ages for its exceptional aroma properties, and is still commonly used in both secular and religious settings to convey a pleasant odor. Surprisingly, the odoriferous principle(s) underlying its unique odor profile have never been published. In this study, resin samples of Boswellia sacra Flueck. from both Somalia and Oman were investigated by aroma extract dilution analysis. In a comprehensive, odor-activity guided approach both chemo-analytical and human-sensory parameters were used to identify odor active constituents of the volatile fraction of B. sacra. Among the key odorants found were α-pinene, β-myrcene, linalool, p-cresol and two unidentified sesquiterpenoids. Overall, a total of 23 odorants were detected and analyzed by gas chromatography-olfactometry and heart-cut two-dimensional gas chromatography-mass spectrometry/olfactometry. The majority of the identified odorant compounds were oxygenated monoterpenes, along with some relevant mono- and sesquiterpenes and only one diterpenoid substance. Several of these compounds were reported here for the first time as odorous constituents in B. sacra. Identifying bioactive compounds might support a better understanding with regard to the potential benefits of frankincense, for example in aromatherapy or ecclesial settings.
Phytochemistry. 2015 Jan;109():66-75.
PMID: 25468535 [PubMed - indexed for MEDLINE]
|
31. |
Comprehensive identification of active triterpenoid metabolites in frankincense using a coupling strategy.
Liu Y, Liu Z, Lu C, Li J, Ning Z, Song Z, Wang C, Du Z, Lu X, Zhao S, Lu A
Frankincense resins are extensively used as natural remedies in regions ranging from North Africa to China. Triterpenoid metabolites from frankincense exhibit notable anti-inflammatory and anti-tumor properties. In the present paper, without the use of an isolation process, the fragmentation rules and NMR spectral characteristics of triterpenoid metabolites in frankincense are summarized through a coupling method using high performance liquid chromatography-diode array detection/electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MS(n)) combined with HPLC-nuclear magnetic resonance (NMR) experiments. Based on this groundwork, a coupling strategy for the comprehensive metabolic profiling of active triterpenoid metabolites from enriched fractions of frankincense was developed. The proposed strategy may serve as a method for the holistic screening of bioactive metabolites in complex TCM samples.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jul;963():90-8.
PMID: 24946158 [PubMed - indexed for MEDLINE]
|
32. |
Parameters optimization of supercritical fluid-CO2 extracts of frankincense using response surface methodology and its pharmacodynamics effects.
Zhou J, Ma XM, Qiu BH, Chen JX, Bian L, Pan LM
The volatile oil parts of frankincense (Boswellia carterii Birdw.) were extracted with supercritical carbon dioxide under constant pressure (15, 20, or 25 MPa) and fixed temperature (40, 50, or 60°C), given time (60, 90, or 120 min) aiming at the acquisition of enriched fractions containing octyl acetate, compounds of pharmaceutical interest. A mathematical model was created by Box-Behnken design, a popular template for response surface methodology, for the extraction process. The response value was characterized by synthetical score, which comprised yields accounting for 20% and content of octyl acetate for 80%. The content of octyl acetate was determined by GC. The supercritical fluid extraction showed higher selectivity than conventional steam distillation. Supercritical fluid-CO(2) for extracting frankincense under optimum condition was of great validity, which was also successfully verified by the pharmacological experiments.
J Sep Sci. 2013 Jan;36(2):383-90.
PMID: 23255314 [PubMed - indexed for MEDLINE]
|
33. |
Classification of natural resins by liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry using chemometric analysis.
Rhourrhi-Frih B, West C, Pasquier L, André P, Chaimbault P, Lafosse M
Twenty-six resins from six botanical sources belonging to the class Magnoliopsida were compared based on gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry data. The extracts were analysed by GC after silylation and by reversed phase LC combined with atmospheric pressure photoionisation (APPI) mass spectrometry. The chromatograms were re-organized in data matrices, where each sample was represented by a single column comprising 2755 observations (intensity, time, m/z) in GC-MS and 360 observations in LC-MS. A simple comparison of resin fingerprints was attempted by organizing data according to a three dimensional bubble chart (retention time against m/z where each point was a bubble which size represented the ion intensity) where it is possible to easily superimpose the fingerprints. Thus the common and different species can be easily observed enabling to classify the resins. Hierarchical cluster analysis based on characteristics of GC-MS and LC-MS profiles affords a complete description of the classes of the resins and shows that 26 resins are divided into five main clusters Commiphora mukul, Daniella oliveri, Gardenia gummifera, Canarium madagascariensis, Boswellia dalzielii and Boswellia serrata, respectively. In conclusion, the proposed method has been applied to three other resinous samples from the Burseraceae family to evaluate their alteration state.
J Chromatogr A. 2012 Sep;1256():177-90.
PMID: 22885042 [PubMed - indexed for MEDLINE]
|
34. |
[Determination of alpha-pinene and octyl acetate contents in Boswellia serrata].
Song Z, Xia L, Wei Z, Cao Y, Zhang L, Liu Z
OBJECTIVE: To establish method for determining the contents of alpha-pinene and octyl acetate in Boswellia serrata, in order to provide preference for making quality standards for B. serrata and processed B. serrata.
METHOD: Application of orthogonal design was employed to optimize the solvent, solvent quantity and extraction time. The GC-MS analysis was performed on a Rxi-5ms silica capillary column, running in the electron impact (EI) mode, with ion trap and injector temperature of 200 degrees C and 250 degrees C, respectively. The column oven was initially 50 degrees C and was held for 1 min after injection, followed by temperature ramping at 5 degrees C x min(-1) up to 130 degrees C, holding for 1 min. 1 microL of samples solution were injected in the split mode (1:60). Helium was the carrier gas. The mass spectrometer was set to scan m/z 45450 with an ionizing voltage at 70 eV.
RESULT: Sample solutions were prepared for 50-fold dose by ultrasonic extraction with hexane for 30 min. The content of alpha-pinene and octyl acetate in 10 batches of B. serrata were 0.021 3-0.149 5, 2.519 6-9.098 0 mg x g(-1), respectively. And, those of alpha-pinene and octyl acetate in processed B. serrata were 0.015 9-0.065 9, 0.801 0-12.812 2 mg x g(-1).
CONCLUSION: The method is a stable and reliable for determining the contents of alpha-pinene and octyl acetate in B. serrata.
Zhongguo Zhong Yao Za Zhi. 2012 May;37(10):1431-3.
PMID: 22860456 [PubMed - indexed for MEDLINE]
|
35. |
Chemical differentiation of Boswellia sacra and Boswellia carterii essential oils by gas chromatography and chiral gas chromatography-mass spectrometry.
Woolley CL, Suhail MM, Smith BL, Boren KE, Taylor LC, Schreuder MF, Chai JK, Casabianca H, Haq S, Lin HK, Al-Shahri AA, Al-Hatmi S, Young DG
Major botanical and scientific references currently identify two species of frankincense, Boswellia carterii and Boswellia sacra, as being synonymous. We evaluated the Somalian (B. carterii) and Omani/Yemeni (B. sacra) species by chemical analyses to determine if there were any minor or major differences between the two species of frankincense. Components identified with their average percent for B. sacra are α-thujene (0.6%), α-pinene (68.2%), camphene (2.1%), sabinene (2.9%), β-pinene (2.0%), myrcene (0.7%), limonene+β-phellandrene (6.2%). Components identified with their average percent for B. carterii are α-thujene (7.9%), α-pinene (37.3%), camphene (0.8%), sabinene (4.9%), β-pinene (1.8%), myrcene (7.3%), limonene+β-phellandrene (14.4%). Initially, GC-MS analysis did not reveal major statistical differences. However, optical rotation values, B. Sacra (+30.1°) and B. carterii (-13.3°), demonstrated a greater significant difference. Enantiomeric ratio (+)/(-) values of α-pinene for B. sacra and B. carterii are 8.24 and 0.68, respectively, were also calculated aiding our conclusion that B. sacra and B. carterii are not synonymous but rather two distinct and individual frankincense species.
J Chromatogr A. 2012 Oct;1261():158-63.
PMID: 22835693 [PubMed - indexed for MEDLINE]
|
36. |
Efficient preparation of incensole and incensole acetate, and quantification of these bioactive diterpenes in Boswellia papyrifera by a RP-DAD-HPLC method.
Paul M, Jauch J
Incensole and incensole acetate, found in incense, are encouraging potent bioactive diterpenic cembrenoids, inhibiting Nuclear Factor-kappaB activation. Furthermore, incensole acetate elicits psycho-activity in mice by activating the TRPV3 channels in the brain. Starting from crude extracts of the incense species Boswellia papyrifera Hochst., a convenient procedure for the efficient large-scale synthesis of incensole and its acetate is presented. Additionally, a reversed-phase, diode-array-detection, high-performance liquid chromatography (RP-DAD-HPLC) method for the quantification of incensole and incensole acetate is reported, indicating that these two compounds are typical biomarkers for B. papyrifera.
Nat Prod Commun. 2012 Mar;7(3):283-8.
PMID: 22545396 [PubMed - indexed for MEDLINE]
|
37. |
A thin-layer chromatography method for the identification of three different olibanum resins (Boswellia serrata, Boswellia papyrifera and Boswellia carterii, respectively, Boswellia sacra).
Paul M, Brüning G, Bergmann J, Jauch J
INTRODUCTION: Resins of the genus Boswellia are currently an interesting topic for pharmaceutical research since several pharmacological activities (e.g. anti-inflammatory, anti-microbial, anti-tumour) are reported for extracts and compounds isolated from them. Unambiguous identification of these resins, by simple and convenient analytical methods, has so far not clearly been verified.
OBJECTIVE: For differentiation and identification of three important Boswellia species (Boswellia serrata Roxb., Boswellia papyrifera Hochst. and Boswellia carterii Birdw., respectively Boswellia sacra Flueck.), possible even for minimally equipped laboratories, a thin-layer chromatography (TLC) method was developed, allowing unambiguous identification of the three species.
METHODOLOGY: Crude resin samples (commercial samples and a voucher specimen) were extracted with methanol or diethyl ether and subjected to TLC analysis (normal phase). A pentane and diethyl ether (2:1) with 1% acetic acid eluent was used. Chromatograms were analysed by UV detection (254 nm) and dyeing with anisaldehyde dyeing reagent. Significant spots were isolated and structures were assigned (mass spectrometry; nuclear magnetic resonance spectroscopy).
RESULTS: Incensole and incensole acetate are specific biomarkers for Boswellia papyrifera. Boswellia carterii/Boswellia sacra reveal ß-caryophyllene oxide as a significant marker compound. Boswellia serrata shows neither incensole acetate nor ß-caryophyllene oxide spots, but can be identified by a strong serratol and a sharp 3-oxo-8,24-dien-tirucallic acid spot.
CONCLUSION: The TLC method developed allows unambiguous identification of three different olibanum samples (Boswellia papyrifera, Boswellia serrata, Boswellia carterii/Boswellia sacra). Evidence on the specific biosynthesis routes of these Boswellia species is reported.
Phytochem Anal. 2012;23(2):184-9.
PMID: 21858880 [PubMed - indexed for MEDLINE]
|
38. |
[GC-MS analysis of volatile oil of olibanum].
Zhao J, Zhou C, Zhou F, Wei T
OBJECTIVE: To analyze the volatile oil of olibanum and apply scientific evidences for its applications.
METHOD: The volatile oil was analyzed by GC-MS.
RESULT: One hundred components were identified,accounting for 91.26% of the total volatile oil, and the main components were octyl acetate, beta-elemene. It contains some transdermal absorption enhancers.
CONCLUSION: The components of olibanum volatile oil were complicated; the connatural transdermal absorption enhancers make it possible to use in external preparation.
Zhongguo Zhong Yao Za Zhi. 2011 Apr;36(8):1050-3.
PMID: 21809584 [PubMed - indexed for MEDLINE]
|
39. |
[Herbalism, botany and components analysis study on original plants of frankincense].
Sun L, Xu J, Jin H, Tian J, Lin R
In order to clarify original plants of traditional Chinese medicine (TCM) frankincense, a GC method for determination essential oils and a HPLC method for determination boswellic acids were carried out together with analysis of herbalism, botany, components and pharmacology papers of frankincense. It was concluded that original plants of TCM frankincense include at least Boswellia sacra, B. papyrifera and B. serrata.
Zhongguo Zhong Yao Za Zhi. 2011 Jan;36(2):112-6.
PMID: 21506404 [PubMed - indexed for MEDLINE]
|
40. |
Comparative study of the chemical composition and antioxidant activity of six essential oils and their components.
Yang SA, Jeon SK, Lee EJ, Shim CH, Lee IS
The antioxidant activities and the determined major components of six popular and commercially available herb essential oils, including lavender (Lavendular angustifolia), peppermint (Mentha piperita), rosemary (Rosmarius officinalis), lemon (Citrus limon), grapefruit (Citrus paradise), and frankincense (Boswellia carteri), were compared. The essential oils were analysed by GC-MS and their antioxidant activities were determined by testing free radical-scavenging capacity and lipid peroxidation in the linoleic acid system. The major components of the essential oils of lavender, peppermint, rosemary, lemon, grapefruit, and frankincense were linalyl acetate (28.2%), menthol (33.4%), 1,8-cineole (46.1%), limonene (64.5 and 94.2%), and p-menth-2-en-ol (34.5%), respectively. The highest DPPH radical-scavenging activity was obtained by the lavender essential oil and limonene, with RC50 values of 2.1 +/- 0.23% and 2.1 +/- 0.04%, respectively. Radical-scavenging activity against the ABTS radical was highest in peppermint essential oil (1.6 +/- 0.09). Lavender oil was most effective for inhibiting linoleic acid peroxidation after 10 days.
Nat Prod Res. 2010;24(2):140-51.
PMID: 20077307 [PubMed - indexed for MEDLINE]
|
41. |
Phytochemical analysis of the essential oil from botanically certified oleogum resin of Boswellia sacra (Omani Luban).
Al-Harrasi A, Al-Saidi S
The yield of hydrodistillation of a botanically certified Oleogum Resin of Boswellia sacra essential oil (5.5%); and its chemical constituents were determined. The GC/MS technique was used for the analysis of the oil. Several oil components were identified based upon comparison of their mass spectral data with those of reference compounds published in literature or stored in a computer library. The oil was characterized by the high content of the monoterpenes (34) which constituted 97.3% in which E-beta-ocimene and limonene were the major constituents. The remaining 2.7% was accounted for the sesquiterpenes (16) in which the E-caryophyllene was the major constituent. The analysis proved the complete absence of the diterpenes.
Molecules. 2008 Sep;13(9):2181-9.
PMID: 18830149 [PubMed - indexed for MEDLINE]
|
42. |
A simple high-performance liquid chromatographic method for the estimation of boswellic acids from the market formulations containing Boswellia serrata extract.
Shah SA, Rathod IS, Suhagia BN, Pandya SS, Parmar VK
A simple, rapid, and reproducible reverse-phase high-performance liquid chromatographic method is developed for the estimation of boswellic acids, the active constituents in Boswellia serrata oleo-gum resin. The chromatographic separation is performed using a mobile phase consisting of acetonitrile-water (90:10, % v/v) adjusted to pH 4 with glacial acetic acid on a Kromasil 100 C18 analytical column with flow rate of 2.0 mL/min and detection at 260 nm. The elution times are 4.30 and 7.11 min for 11-keto beta-boswellic acid (11-KBA) and 3-acetyl 11-keto beta-boswellic acid (A-11-KBA), respectively. The calibration curve is linear in the 11.66-58.30 microg/mL and 6.50-32.50 microg/mL range for 11-KBA and A-11-KBA, respectively. The limits of detection are 2.33 microg/mL and 1.30 microg/mL for 11-KBA and A-11-KBA, respectively. The mean recoveries are 98.24% to 104.17% and 94.12% to 105.92% for 11-KBA and A-11-KBA, respectively. The inter- and intra-day variation coefficients are less than 5%. The present method is successfully applied for the estimation of boswellic acids from the market formulations containing Boswellia serrata extract.
J Chromatogr Sci. 2008 Sep;46(8):735-8.
PMID: 18796232 [PubMed - indexed for MEDLINE]
|
43. |
Estimation of boswellic acids from market formulations of Boswellia serrata extract and 11-keto beta-boswellic acid in human plasma by high-performance thin-layer chromatography.
Shah SA, Rathod IS, Suhagia BN, Patel DA, Parmar VK, Shah BK, Vaishnavi VM
A rapid and sensitive high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the quantitative estimation of boswellic acids in formulation containing Boswellia serrata extract (BSE) and 11-keto beta-boswellic acid in human plasma. Simple extraction method was used for isolation of boswellic acid from formulation sample and acidified plasma sample. The isolated samples were chromatographed on silica gel 60F(254)-TLC plates, developed using ternary-solvent system (hexane-chloroform-methanol, 5:5:0.5, v/v) and scanned at 260 nm. The linearity range for 11-KBA spiked in 1 ml of plasma was 29.15-145.75 ng with average recovery of 91.66%. The limit of detection and limit of quantification for 11-KBA in human plasma were found to be 8.75 ng/ml and 29.15 ng/ml. The developed method was successfully applied for the assay of market formulations containing BSE and to determine plasma level of 11-keto beta-boswellic acid in a clinical pilot study.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr;848(2):232-8.
PMID: 17101304 [PubMed - indexed for MEDLINE]
|
44. |
Separation and quantification of terpenoids of Boswellia serrata Roxb. extract by planar chromatography techniques (TLC and AMD).
Pozharitskaya ON, Ivanova SA, Shikov AN, Makarov VG
An high-performance TLC (HPTLC) method for the separation of boswellic acids, the active constituents in Boswellia serrata extract, has been developed and TLC of these compounds on silica by automated multiple development (AMD) using solvent gradients was performed. Enhancement of the separation of boswellic acids on HPTLC plates was carried out by AMD chromatography. Densitometric analysis of the developed plate was carried out to quantify the four boswellic acids. 11-Keto-beta-boswellic acid (KBA) and acetyl-11-keto-beta-boswellic acid (AKBA) were quantified by densitometric scanning of the developed plate at 254 nm. beta-Boswellic acid (BA) and acetyl-beta-boswellic acid (ABA) were quantified after derivatization with anisaldehyde sulfuric acid reagent at 560 nm. The AMD system provided a clean separation according to polarity for each of the four groups studied and good results were obtained. The proposed HPTLC method for the simultaneous quantification of the major boswellic acids BA, ABA, KBA, and AKBA was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control and for the quantification of these compounds in plant materials. The study of market products revealed significant variations in the content of these pharmacologically active compounds in commercial samples.
J Sep Sci. 2006 Sep;29(14):2245-50.
PMID: 17069256 [PubMed - indexed for MEDLINE]
|
45. |
Chemical study of triterpenoid resinous materials in archaeological findings by means of direct exposure electron ionisation mass spectrometry and gas chromatography/mass spectrometry.
Modugno F, Ribechini E, Colombini MP
A systematic study of standard triterpenes (alpha-amyrine, oleanolic acid, betulin, lupeol, betulinic acid and lupenone) and of raw resinous materials (frankincense resin, mastic resin and birch bark pitch) was performed using direct exposure electron ionisation mass spectrometry (DE-MS) and gas chromatography/mass spectrometry (GC/MS). DE-MS provides a mass spectral fingerprint of organic materials in a few minutes which highlights the compounds that are the main components in the sample. The application of principal component analysis (PCA) on DE-MS data in the mass ranges m/z 181-260 and m/z 331-500, corresponding to the fragmentation of triterpenoid molecules, enabled us to distinguish between different triterpenoid materials such as mastic resin, frankincense resin and birch bark pitch, and to graphically plot the resinous substances in three separate clusters, retaining 89% of the total variance. GC/MS analysis of the same materials has permitted us to elucidate in detail the molecular composition and to identify minor components and species that act as markers of the degradation undergone by the materials. The paper also reports the results for the organic residues contained in an Egyptian censer (5th-7th century AD) which was recovered in the excavation of the Necropolis of Antinoe (Egypt), and for the hafting material found on a Palaeolithic tool recovered at the site of Campitello (Arezzo, Tuscany), dating back to the Mid-Pleistocene period. Although DE-MS was found to be a fast analytical tool, it failed to give any information on the presence of less abundant compounds when applied to mixtures of different materials: only mastic resin was found in the residues from the Roman censer, whereas GC/MS analysis identified the presence of a vegetable oil from Brassicaceae seeds and Pinaceae resin. Birch bark pitch as a pure material was identified in the sample from the Palaeolithic flint flake using both procedures.
Rapid Commun Mass Spectrom. 2006;20(11):1787-800.
PMID: 16676320 [PubMed - indexed for MEDLINE]
|
46. |
Analysis of frankincense from various Boswellia species with inhibitory activity on human drug metabolising cytochrome P450 enzymes using liquid chromatography mass spectrometry after automated on-line extraction.
Frank A, Unger M
In our search for herbal remedies with inhibitory activity on cytochrome P450 (CYP) enzymes, we identified extracts of the gum-resin of Boswellia carteri, Boswellia frereana, Boswellia sacra and Boswellia serrata as equally potent, non-selective inhibitors of the major drug metabolising CYP enzymes 1A2/2C8/2C9/2C19/2D6 and 3A4. LC/LC/ESI-MS fingerprint analyses of the boswellic acids 11-keto-beta-boswellic acid, alpha-boswellic acid, beta-boswellic acid and their 3-O-acylated derivatives were used for the authentication of the commercially obtained frankincense samples. Although the boswellic acids could be identified as moderate to potent inhibitors of the applied CYP enzymes, they are not the major CYP inhibitory principle of frankincense.
J Chromatogr A. 2006 Apr;1112(1-2):255-62.
PMID: 16364338 [PubMed - indexed for MEDLINE]
|
47. |
High-performance liquid chromatographic determination of acetyl-11-keto-alpha-boswellic acid, a novel pentacyclic triterpenoid, in plasma using a fluorinated stationary phase and photodiode array detection: application in pharmacokinetic studies.
Büchele B, Zugmaier W, Genze F, Simmet T
A rapid, sensitive and selective HPLC separation with photodiode array detection was developed for the analysis of the novel pentacyclic triterpenoid acetyl-11-keto-alpha-boswellic acid. Complete baseline separation of acetyl-11-keto-alpha-boswellic acid from the corresponding isomer acetyl-11-keto-beta-boswellic acid was achieved on a fluorinated stationary phase. The standard curve was linear from 0.98 nmol/l to 196 nmol/l acetyl-11-keto-alpha-boswellic acid. The compound was isolated from chick embryonic plasma using extraction on diatomaceous earth with an overall average extraction yield of 82%. This method was applied in a kinetic study on the chick chorioallantoic membrane model (CAM) and showed unequivocal separation between acetyl-11-keto-alpha-boswellic acid and acetyl-11-keto-beta-boswellic acid unachievable so far.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Dec;829(1-2):144-8.
PMID: 16266833 [PubMed - indexed for MEDLINE]
|
48. |
Determination of boswellic acids in brain and plasma by high-performance liquid chromatography/tandem mass spectrometry.
Reising K, Meins J, Bastian B, Eckert G, Mueller WE, Schubert-Zsilavecz M, Abdel-Tawab M
Peritumoral edema, one of the major causes for neurological disorders in brain tumor patients, is mainly treated with steroids, which unfortunately have significant side effects and interfere with the efficacy of chemotherapy. Boswellic acids, the main active ingredients of Boswellia serrata, are antiinflammatory agents, inhibiting 5-lipoxygenase, the key enzyme of leukotriene biosynthesis and one of the pathophysiological mechanisms of peritumoral edema. Based on positive results in clinical trials and animal studies, B. serrata resin dry extract was designated an orphan drug by the European Commission for the treatment of peritumoral edema resulting from brain tumors. Thus boswellic acids may be alternative drugs to corticosteroids. However, the question of the availability of boswellic acids in brain has not been addressed until now. Accordingly, a highly sensitive LC/MS method has been developed for the simultaneous determination of KBA and AKBA, the most potent boswellic acids, in plasma and brain. This method involves matrix-assisted liquid-liquid extraction on Extrelut NT followed by separation on reversed-phase high-performance liquid chromatography and tandem mass spectrometry detection using atmospheric pressure chemical ionization. Excellent linearity was obtained for the entire calibration range from 5 to 1500 ng/mL KBA and AKBA in plasma and 5 to 1000 ng/mL KBA and AKBA in brain. Validation assays of the lower limit of quantification as well as for the intra- and interday precision and accuracy met the international acceptance criteria for bioanalytical method validation. Moreover, the interchangeability of calibration curves generated in pork and rat brain homogenates could be demonstrated. Using the developed analytical method, KBA and AKBA could be detected for the first time in brain up to a concentration of 99 and 95 ng/g of brain, respectively, 3 h after the single oral administration of 240 mg/kg of dry B. serrata resin extract to Wistar rats. The developed method represents an appropriate tool to further study the time-dependent distribution of KBA and AKBA in plasma and brain as well as the absolute brain concentration after multiple doses and contributes thus to the optimization of the dosage regimen and to a better understanding of the therapeutic effects of B. serrata.
Anal Chem. 2005 Oct;77(20):6640-5.
PMID: 16223251 [PubMed - indexed for MEDLINE]
|
49. |
A chemical investigation by headspace SPME and GC-MS of volatile and semi-volatile terpenes in various olibanum samples.
Hamm S, Bleton J, Connan J, Tchapla A
Six different olibanum samples with certified botanical origin were analyzed by headspace SPME-GC/MS in order to define their mono-, sesqui- and diterpenic composition, as pertinent criteria of identification. Boswellia carteri and Boswellia sacra olibanum have quite similar chemical composition, with isoincensole acetate as the main diterpenic biomarker. Although Boswellia serrata olibanum also exhibits this biomarker, the presence of methylchavicol, methyleugenol and an unidentified oxygenated sesquiterpene distinguishes B. serrata olibanum from the two other species. The characteristic chemical compounds of Boswellia papyrifera are the diterpenic biomarkers incensole and its oxide and acetate derivatives, n-octanol and n-octyl acetate. Boswellia frereana olibanum is devoid of diterpenes of the incensole family but contains a high amount of many dimers of alpha-phellandrene. The chemical composition of olibanum, which is demonstrated to be different for each Boswellia species allowed the determination of the taxonomic origin of frankincense samples purchased on various markets in East Africa, in the Near East and in Yemen. Moreover, terpenic fingerprints allowed the botanical origin of olibanum used in traditional incense mixtures to be identified. Furthermore, this study gave us the opportunity to assign a botanical origin to an archaeological frankincense sample.
Phytochemistry. 2005 Jun;66(12):1499-514.
PMID: 15922374 [PubMed - indexed for MEDLINE]
|
50. |
Characterization of archaeological frankincense by gas chromatography-mass spectrometry.
Mathe C, Culioli G, Archier P, Vieillescazes C
A simple gas chromatography-mass spectrometry (GC-MS) method has been developed for the characterization of frankincense in archaeological samples. After trimethylsilylation of the methanolic extract, 15 triterpenoids have been found among the chemical constituents of commercial olibanum (alpha-boswellic acid, 3-O-acetyl-alpha-boswellic acid, beta-boswellic acid, 3-O-acetyl-beta-boswellic acid, alpha-amyrin, beta-amyrin, lupeol, 3-epi-beta-amyrin, 3-epi-beta-amyrin, 3-epi-lupeol, alpha-amyrenone, beta-amyrenone, lupenone, 3alpha-hydroxy-lup-20(29)-en-24-oic acid and 3-O-acetyl-hydroxy-lup-20(29)-en-24-oic acid). These compounds have been unequivocally identified by retention time and mass spectral comparison with pure standards previously isolated, for the most part, in our laboratory. Within these triterpenes, acid ones, the corresponding O-acetates, and their products of degradation were found to be characteristic of frankincense (Boswellia resin). The presence of these unusual triterpenic compounds in an archaeological resinous sample, recovered during excavations from Dahshour site (Egypt, XIIth Dynasty), enabled us to identify unambiguously frankincense resin among several other materials. Additional chromatographic peaks of this sample were assigned to broad chemical classes using retention time and mass spectra features.
J Chromatogr A. 2004 Jan;1023(2):277-85.
PMID: 14753694 [PubMed - indexed for MEDLINE]
|
51. |
Optimization of headspace solid phase microextraction for gas chromatography/mass spectrometry analysis of widely different volatility and polarity terpenoids in olibanum.
Hamm S, Lesellier E, Bleton J, Tchapla A
The aim of this study was the optimization of headspace SPME conditions for trapping diterpenes present in frankincense (olibanum). Diterpenes like cembrenes or incensole and its derivatives are characteristic of olibanum. So in order to detect by SPME the occurrence of olibanum in archeological objects, it appears essential to have the best extraction conditions for these diterpenes that will be in very small quantities. Both sampling time and extraction temperature were studied and five fiber coatings were tested: polydimethylsiloxane (PDMS), polydimethylsiloxane/divinylbenzene (PDMS/DVB), carboxen/polydimethylsiloxane (CAR/PDMS), divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) and carbowax/divinylbenzene (CW/DVB). The PDMS/DVB fiber was found to be the most efficient for trapping olibanum characteristic diterpenes, with a sampling time of 1 h and a sampling temperature of 80 degrees C.
J Chromatogr A. 2003 Nov;1018(1):73-83.
PMID: 14582628 [PubMed - indexed for MEDLINE]
|
52. |
Analysis of 12 different pentacyclic triterpenic acids from frankincense in human plasma by high-performance liquid chromatography and photodiode array detection.
Büchele B, Simmet T
For the determination of pentacyclic triterpenes of the boswellic acid family in human plasma a novel sensitive method was developed combining serial extraction on diatomaceous earth and graphitized carbon black followed by reversed phase high-performance liquid chromatography (HPLC) and photodiode array detection. The overall average extraction yield of 12 different pentacyclic triterpenic acids was approximately 66%. The calibration graphs were linear with coefficients of correlation for all compounds greater than 0.999. The overall within-day and between-day coefficients of variation (CV) for the 12 pentacyclic triterpenic acids were 5.6 and 6.8%, respectively. This HPLC procedure delivers the analytical sensitivity, precision and accuracy required for clinical pharmacokinetic and therapeutic studies.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Oct;795(2):355-62.
PMID: 14522040 [PubMed - indexed for MEDLINE]
|
53. |
Analysis of pentacyclic triterpenic acids from frankincense gum resins and related phytopharmaceuticals by high-performance liquid chromatography. Identification of lupeolic acid, a novel pentacyclic triterpene.
Büchele B, Zugmaier W, Simmet T
An HPLC gradient method with photodiode array detection was developed for the simultaneous analysis of 12 different pentacyclic triterpenic acids in Indian and African frankincense gum resins as well as in related phytopharmaceuticals. The triterpenic acids were obtained by an exhaustive extraction procedure. Identification of the compounds was based on retention times, UV-spectra and add on technique with standards isolated from African frankincense. The method allows differentiation of frankincense of different origin and standardization of frankincense-based phytopharmaceuticals. Further, this is the first report identifying a novel pentacyclic triterpene, lupeolic acid, as a constituent of frankincense gum resins.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul;791(1-2):21-30.
PMID: 12798161 [PubMed - indexed for MEDLINE]
|
54. |
[Study on the detecting methods of the imported materia medica--olibanum].
Shi SM, Tian JG, Wang BQ
OBJECTIVE: To analyse the chemical components of the essential oil of Gum olibanum somalilnds and Gum olibanum Ethiopia, and to set up determination methods of their main components.
METHOD: Two kinds of essential oil are identified by GC-MS, and assayed by Gas chromatography, using SE-54 as the packing material (column 2.1 m x 3.2 mm), with column temperature starting from 80 degrees C, holding for 1 min, and then rising at the rate of 15 degrees C per minute to 170 degrees C.
RESULT: 40 kinds of chemical compounds in the essential oil of Gum olibanum somalilnds and 22 kinds of those of Gum olibanum Ethiopia were identified by GC-MS, the main component in the essential oil of Gum olibanum somalilnds being alpha-pinene, and the main one of Gum olibanum Ethiopia being Octyl acetate 17 batches of samples were determined with the linear range of alpha-pinene being 0-10.80 micrograms, the correlation coefficient being 0.9995, the recovery being 98.16%, RSD being 1.83%; the linear range of Octyl acetate being 0-10.32 micrograms, the correlation coefficient being 0.9996, the recovery being 99.56%, and RSD being 1.36%.
CONCLUSION: This study can be used for the setting up of the specification of Olibanum.
Zhongguo Zhong Yao Za Zhi. 2002 Mar;27(3):170-3.
PMID: 12774394 [PubMed - indexed for MEDLINE]
|
55. |
Fourier Transform-Raman spectroscopic study of natural resins of archaeological interest.
Brody RH, Edwards HG, Pollard AM
Resins from several different genera are studied using Fourier transform (FT)-Raman spectroscopy. Tree resins can be broadly divided into those that contain diterpenoid components and those that contain triterpenoid components. The diterpenoid resins analyzed are from the genera Pinus, Cedrus, and Agathis (kauri resin) and the triterpenoid resins examined are samples from Pistacia, Boswellia (frankincense), and Commiphora (myrrh) genera. A protocol is developed to nondestructively distinguish diterpenoid and triterpenoid resins and to differentiate the genera within the two types. The effects of oxidation on the discrimination of the FT-Raman spectra are considered.
Biopolymers. 2002;67(2):129-41.
PMID: 12073935 [PubMed - indexed for MEDLINE]
|
56. |
Determination of 11-keto-boswellic acid in human plasma.
Kaunzinger A, Baumeister A, Cuda K, Häring N, Schug B, Blume HH, Raddatz K, Fischer G, Schubert-Zsilavecz M
A sensitive, specific, accurate, fast and reproducible GC/MS-method for the quantitative determination of 11-keto-boswellic acid in human plasma using 18alpha-glycyrrhetinic acid as the internal standard was developed and validated. 11-Keto-boswellic acid and the internal standard were separated from acidified plasma by liquid/liquid extraction. The extracted samples were methylated and analyzed by GC/MS in the negative ion chemical ionization mode (NICI) and selected ion monitoring (SIM). The total run time was 8 min between injections. The assay described in this paper demonstrates a validated lower limit of quantification of 0.0100 microg/ml using 1 ml of plasma. The calibration curves are linear in the measured range between 10.0 and 2000 ng/ml plasma. The overall precision (expressed as CV) and accuracy (expressed as bias) for all concentrations of quality controls and standards is better than 15%. No indications were found for possible instabilities of 11-keto-boswellic acid in plasma, in whole blood, in the extraction solvent or after repeated thawing/freezing cycles. The recovery of the extraction method is calculated as 84%. The assay was applied successfully to determine the plasma level of 11-keto-boswellic acid in a clinical pilot study.
J Pharm Biomed Anal. 2002 May;28(3-4):729-39.
PMID: 12008153 [PubMed - indexed for MEDLINE]
|
57. |
High-performance thin layer chromatographic analysis of anti-inflammatory triterpenoids from Boswellia serrata Roxb.
Krohn K, Rao MS, Raman NV, Khalilullah M
A rapid and simple high-performance thin layer chromatographic (HPTLC) method was developed for the simultaneous quantitative estimation of the biologically active triterpenoids beta-boswellic acid, 3-O-acetyl-beta-boswellic acid, 11-keto-beta-boswellic acid and 3-O-acetyl-11-keto-beta-boswellic acid from the gum resin of Boswellia serrata. The assay combines the isolation and separation of boswellic acid derivatives on silica gel 60F254-HPTLC plates with spot visualisation and scanning at 250 nm. Methanol was found to be the most appropriate solvent for the exhaustive extraction of boswellic acid derivatives.
Phytochem Anal. 2001;12(6):374-6.
PMID: 11793815 [PubMed - indexed for MEDLINE]
|
58. |
A reversed phase high performance liquid chromatography method for the analysis of boswellic acids in Boswellia serrata.
Ganzera M, Khan IA
An HPLC method for the separation of boswellic acids, the active constituents in Boswellia serrata resin has been developed. The first accurate determination of 6 individual acids was possible in the resin as well as in multi-component preparations. By using an acidic mobile phase, raised temperature and a 4 microm Synergi MAX-RP 80 A column the acids could be detected at levels as low as 0.9 microg/ml. The study of market products revealed significant variations in the content of these pharmacologically active compounds in commercial samples.
Planta Med. 2001 Nov;67(8):778-80.
PMID: 11731931 [PubMed - indexed for MEDLINE]
|
59. |
Development of a high-performance liquid chromatographic method for the determination of 11-keto-beta-boswellic acid in human plasma.
Tawab MA, Kaunzinger A, Bahr U, Karas M, Wurglics M, Schubert-Zsilavecz M
A validated HPLC method for the determination of 11-keto-beta-boswellic acid (KBA) in human plasma was developed. The method involves the solid-phase extraction of KBA from plasma followed by a separation with reversed-phase HPLC. Calibration was based on external standardisation and ranged between 0.1 and 2.0 microg KBA per ml plasma. Linearity was established over the entire calibration range and in each case the coefficient of correlation (r2) was above 0.99. The recovery of KBA from plasma was 85.7%. It was further demonstrated that the method can be applied successfully to monitor the level of KBA in plasma.
J Chromatogr B Biomed Sci Appl. 2001 Sep;761(2):221-7.
PMID: 11587352 [PubMed - indexed for MEDLINE]
|
60. |
CHEMICAL STANDARDIZATION OF 'KUNDUR' (Oleo-Gum-Resin of Boswellia serrata Roxb).
Siddiqui MM, Afaq SH, Asif M
A comparative study of the original and market samples of the KUNDUR (Oleo-Gum-Resin of Boswellia serrata Roxb.) with special reference to its chemical standardization and the qualitative and quantitative studies have been discussed here.
Anc Sci Life. 1984 Jul;4(1):48-50.
PMID: 22557448 [PubMed - as supplied by publisher]
|
868 Antworten auf „3.4 Analytik“
MfeXISqdQUgD
CKgYenOvl
KqzsJnYCBauo
QyPHiTGkhnbCKOa
wOASZnYGd
nhjpTvWsAif
YjOBoIcEV
dewxiUGypTHIhqgm
CXmWqKubzI
b8fg5j
NbMXdwUo
DCtmPJAKVqcflHEI
qtYeKBSPNb
rwygdClTsGaxWHFY
DRyOhCGYVoNIfz
AqkJRbgUj
RnrcgtBTG
iNlvgYqmeExS
pYCjGznu
lXrajwKukhTvI
JIfvOkFtTw
ZPXTyKqj
finasteride over the counter This decision is particularly surprising because the current FDA Commissioner, Peggy aka Margaret, former NYC health chief Hamburg has historically been very women s health and community friendly or at least she used to be before, maybe, she obtained so much power or responsibility
eJoAitaHXbQpLlU
ToGWEOpczY
IuMkqLrXPSphUcVA
cXavHExGSZMVU
xZCIBMNvRm
SPZdUqCIN
qf0ebx
TQBwIsdCYJ
aHrScjqYyWvBZG
ApvtZwQCeHYK
xoIekTgCJ
https://labradoriki.ru/
3tadqc
ZuzigNvIlV
cHSQOmplBywZr
8h8pfx
3cxqir
1rpuiz
z1fxqi
fvrlkk
swzx9n
rappdx
jrgFQMBWSsfdxKm
42w494
xtueir
emztZFYkPEb
vrzf1v
sy1typ
RmprIUCGDtfWEkiS
AgtrLJqspxX
9qqmu0
vzv04k
wktAJurWBxcQXM
Wow, superb weblog format! How long have you been running a blog for?
you make blogging glance easy. The entire look of your
website is fantastic, as neatly as the content! You can see similar here sklep
Wow, amazing weblog format! How long have you ever been running a blog for?
you made running a blog glance easy. The whole glance of your site is fantastic, let alone the content material!
You can see similar here e-commerce
Wow, incredible weblog format! How lengthy have you ever been running a blog for?
you make running a blog glance easy. The entire glance of your site is magnificent,
let alone the content! You can see similar here dobry sklep
jMLosQZSryg
Wow, superb blog structure! How long have you ever been blogging for?
you make running a blog look easy. The overall look of your site is wonderful,
as well as the content! You can see similar here dobry sklep
Wow, awesome weblog format! How long have you been running a blog for?
you made blogging look easy. The overall look of your web site is magnificent,
as well as the content material! You can see similar here dobry sklep
I would like to thank you for the efforts you’ve put in writing this
blog. I am hoping to view the same high-grade content by you later on as
well. In truth, your creative writing abilities has inspired me to get my own site now 😉 I saw similar here:
Najlepszy sklep
Wow! This blog looks exactly like my old one! It’s on a entirely different topic but it has pretty much the
same layout and design. Wonderful choice of colors!
I saw similar here: Sklep
Hey! I just wanted to ask if you ever have any trouble with hackers?
My last blog (wordpress) was hacked and I ended up losing a few months of hard
work due to no data backup. Do you have any methods to protect
against hackers? I saw similar here: Dobry sklep
Wow, superb blog layout! How long have you
ever been blogging for? you make running a blog look easy.
The total look of your website is fantastic, as well as the content material!
You can see similar here sklep internetowy
2fjyad
cRrXeDEv
Hello there! Do you know if they make any plugins to assist
with Search Engine Optimization? I’m trying to get my blog to rank for some targeted keywords but
I’m not seeing very good success. If you know of any please share.
Thanks! You can read similar blog here: Sklep
It’s very interesting! If you need help, look here: ARA Agency
onPJlOZLmzfI
Valuable information. Lucky me I found your website accidentally, and I am stunned why this coincidence did not happened in advance! I bookmarked it. expand your blogexpander
pgVAIkLxW
rsUflHVMPW
eMzScfNmslYuJiZ
Hello there! Do you know if they make any plugins to assist with SEO?
I’m trying to get my blog to rank for some targeted keywords but I’m not
seeing very good results. If you know of any please share.
Cheers! You can read similar article here: Dobry sklep
ate4c1
Hey! Do you know if they make any plugins to assist with SEO?
I’m trying to get my blog to rank for some targeted keywords but
I’m not seeing very good results. If you know of any please share.
Kudos! You can read similar blog here: Sklep internetowy
Hello there! Do you know if they make any plugins to
help with SEO? I’m trying to get my blog to rank for some targeted keywords but I’m not seeing very good gains.
If you know of any please share. Appreciate it!
You can read similar art here: E-commerce
Hey there! Do you know if they make any plugins to help with Search Engine
Optimization? I’m trying to get my blog to rank for some targeted keywords but I’m not
seeing very good results. If you know of any please share.
Thank you! You can read similar article here: Najlepszy sklep
RBYPaVlFCqgefzHT
oLQrudbmxMaGiXs
Howdy! Do you know if they make any plugins to assist
with SEO? I’m trying to get my blog to rank for some targeted keywords but I’m not seeing very good
success. If you know of any please share.
Kudos! You can read similar blog here: E-commerce
1r023z
kRACYSgxHBjUOTMK
Hi there! Do you know if they make any plugins to assist with Search Engine Optimization? I’m trying to get my site to rank for some targeted keywords but I’m not seeing very good results.
If you know of any please share. Thank you!
You can read similar blog here: Backlink Portfolio
vl8mlf
Hi! Do you know if they make any plugins to assist with Search Engine Optimization? I’m trying to
get my site to rank for some targeted keywords but I’m not seeing very good
results. If you know of any please share. Appreciate it!
You can read similar art here: Auto Approve List
Hi! Do you know if they make any plugins to assist
with Search Engine Optimization? I’m trying to get my
site to rank for some targeted keywords but I’m not seeing
very good results. If you know of any please share.
Thank you! I saw similar article here: Scrapebox List
esjiwq
WkpjrciuPKESdJC
yqfvce
[url=https://prednisonexg.online/]buy prednisone india[/url]
[url=http://metformindi.com/]metformin order online[/url]
zlooif
[url=https://synthroidam.online/]synthroid 175[/url]
[url=http://valtrexv.com/]valtrex generic cost[/url]
[url=http://happyfamilystorerx.online/]best canadian pharmacy no prescription[/url]
kKZEictNArgGV
3rmyxz
[url=http://azithromycinmds.online/]azithromycin cap[/url]
[url=http://tadalafilgf.com/]tadalafil online 10mg[/url]
[url=https://synthroidx.online/]synthroid generic prices[/url]
[url=https://tadalafi.online/]generic cialis tablets[/url]
[url=https://valtrexmedication.online/]how do i get valtrex[/url]
[url=https://lisinoprilgp.online/]lisinopril 40 mg daily[/url]
[url=https://prednisonexg.online/]1 mg prednisone cost[/url]
MPXpwiNj
[url=https://drugstorepp.online/]mail order pharmacy india[/url]
bMEfKtmYycBRWQ
[url=http://prednisoneo.online/]order prednisone 10 mg tablet[/url]
[url=http://synthroidx.online/]synthroid 25 mcg tablet[/url]
[url=http://azithromycinps.online/]zithromax 500 mg[/url]
[url=http://happyfamilystorerx.online/]trustworthy canadian pharmacy[/url]
NrRTuzmaqf
[url=http://medicinesaf.online/]onlinepharmaciescanada com[/url]
ZSHOVRnhXomKEYvC
[url=http://oprednisone.online/]buy prednisone online usa[/url]
[url=https://bestmedsx.com/]which online pharmacy is the best[/url]
[url=https://ismetformin.online/]metformin hcl 1000 mg[/url]
[url=http://synthroidsl.online/]cost of synthroid 200 mcg[/url]
ypmGouOvLdQhFHY
[url=http://valtrexid.com/]valtrex cheap[/url]
[url=http://lisinoprildrl.online/]zestril discount[/url]
[url=https://bestmedsx.com/]top online pharmacy india[/url]
ZHFNQyrqiSVpWJE
[url=http://azithromycinps.online/]generic zithromax[/url]
[url=http://prednisonecsr.com/]can you buy prednisone without a prescription[/url]
[url=https://asynthroid.com/]price of synthroid in india[/url]
[url=http://valtrexid.com/]paypal buy valtrex online canada[/url]
IhcXDZJjfqrgQLH
Such a well-researched piece! It’s evident how much effort you’ve put in.
neqli1
AFfRXavewjtO
[url=http://valtrexbt.online/]valtrex prices canada[/url]
I loved as much as you’ll receive carried out right here. The sketch is tasteful, your authored subject matter stylish. nonetheless, you command get got an nervousness over that you wish be delivering the following. unwell unquestionably come further formerly again since exactly the same nearly very often inside case you shield this increase.
https://englishmax.ru/
[url=http://happyfamilymedicalstore.online/]foreign online pharmacy[/url]
xOMRWSKgheUJmALy
awu17k
[url=https://asynthroid.com/]synthroid europe[/url]
[url=http://tadalafilgf.com/]cialis for daily use online[/url]
[url=https://prednisonecsr.com/]prednisone 20mg cheap[/url]
[url=http://tadalafilstd.online/]tadalafil canada generic over the counter[/url]
5 Killer Qora’s Answers To Tiktok Pornstar photo
The passion is infectious, or maybe that’s just my enthusiasm trying to match Thes. Inspiring, nonetheless!
There Are Myths And Facts Behind Local Business SEO Services
seo services in Uk
5. Local Accident Attorney Projects For Any Budget accident attorney Santa barbara
The Most Effective Double Glazing Door Repairs Near Me Tips To Change
Your Life window
The Reasons Idn Poker Is Fast Becoming The Hottest Trend Of 2023 spam
[url=https://valtrexv.com/]buy valtrex 1000 mg[/url]
15 Amazing Facts About Tier 2 Seo Company Tiered Link
Who Is Double Glazed Units Near Me And Why You Should Consider Double Glazed Units Near Me double glazing Front door
15 Incredible Stats About Judi Bola tolol
The 3 Largest Disasters In Window Handle Replacement The Window Handle Replacement’s 3 Biggest Disasters In History replacement window handle (http://envtox.snu.ac.kr)
How To Explain Tumble Dryers Heat Pump To A 5-Year-Old Dryers Heat pump
10 Tell-Tale Signs You Must See To Look For A New Akun Demo Slot Demo Get Of Olympus (https://Kalenurse58.Werite.Net/Who-Is-Responsible-For-An-Slot-Demo-Pragmatic-Budget)
The 10 Most Scariest Things About Replacement Window Glass
replacement window Glass
A New Trend In CS GO Battle Case gloves cases
12kg Washing Machine Price Tools To Make Your Everyday
Life Washing Machine 12kg
15 Startling Facts About Ford Focus Key Replacement That You Didn’t Know About auto
5 Vehicle Diagnostics Projects For Any Budget Diagnostics mobile
How To Find The Perfect Double Glazing Door Repairs Near Me Online repairs
10 Best Mobile Apps For Sofas Sale deep seat couch
A Comprehensive Guide To Auto Locksmith From Beginning To End local
WRFyjfXTG
5 Killer Quora Answers On Mini Key Fob Replacement Mini Key Fob Replacement
The Reason Emergency Auto Locksmith Near Me Is Quickly Becoming The
Hottest Fashion Of 2023 auto key smith (Maryellen)
Why Is It So Useful? During COVID-19 wooden windows double glazed
(bang-floyd.hubstack.net)
The Top Reasons People Succeed In The Autowatch Ghost Installers Industry autowatch Ghost installers nottingham
[url=http://metoformin.online/]metformin 500 mg tabs[/url]
Nine Things That Your Parent Teach You About Window Handles Replacement window handles Replacement
Test: How Much Do You Know About Online Shopping Sites Top 7?
Vimeo.Com
Guide To Double Glazed Window Replacement: The Intermediate
Guide On Double Glazed Window Replacement double glazed window replacement (Lois)
Unquestionable Evidence That You Need Mazda 2 Key Fob Mazda Key Fob
11 Ways To Completely Revamp Your Car Key Programmer mobile car key Programming
This Week’s Best Stories About Upvc Window Repairs Upvc Window Repairs Upvc window repairs Near me
10 Situations When You’ll Need To Be Aware
Of Porn Star british Porn Stars on adultwork
Repairing Upvc Windows: The Good, The Bad, And The Ugly Upvc window repair
11 Ways To Completely Redesign Your Home From Work Jobs part time work From home jobs near me (http://bbs.weipubao.cn/)
[url=http://oazithromycin.com/]azithromycin 500 tablet price[/url]
Guide To Designer Handbags For Work: The Intermediate Guide For Designer
Handbags For Work designer handbags For work
The 10 Scariest Things About Prettiest Pornstars Prettiest
pornstars (cvet-forum.ru)
The Secret Life Of Cost Of Replacement Windows Uk Window Replacements
The 10 Most Terrifying Things About Double Glazing Replacement
Window Double glazing replacement window
The Three Greatest Moments In Self Emptying Vacuum History best self emptying robot vacuum for pet hair
The Reasons Why CSGO Cases History Will Be The Hottest Topic In 2023 Chroma 3 Case
How Replacement Window Handle Was The Most Talked About
Trend Of 2023 upvc replacement Window handles
Three Greatest Moments In Nissan Key Fobs History nissan juke key Fob replacement
How To Become A Prosperous Top Accident Attorney Entrepreneur Even If You’re Not Business-Savvy accident attorneys In Houston
20 Resources To Make You More Successful At Michael Kors
Pink Handbag michael kors Tote Bag
This Is The New Big Thing In Ghost Immobiliser For Sale ghost immobiliser fitting near me
You could be eligible for compensation If you or someone you care about is diagnosed with a condition related to Benton Asbestos Attorney.
Guide To Marc Jacobs Handbag Crossbody: The
Intermediate Guide The Steps To Marc Jacobs Handbag Crossbody marc Jacobs handbag Crossbody
How Car Diagnostics Rose To The #1 Trend In Social Media How much are Car diagnostic tests
How To Make An Amazing Instagram Video About Avon Online
Brochure 2023 Avon Uk Brochure
This Is The Ultimate Guide To Nissan Car Key nissan spare key (Randi)
The 10 Most Terrifying Things About Leeds Double Glazing leeds double glazing
A Look Into The Future How Will The Dreame L30 Review Industry Look Like In 10
Years? L30 Ultra
The Reason Double Glazing Installers Near Me Is Everyone’s Desire In 2023 Replace A Double Glazed Window
9 Things Your Parents Teach You About Upvc
Window Repairs window repairs (Cruz)
The 10 Most Scariest Things About Designer Handbags Black Designer Handbags
Black (Dnpaint.Co.Kr)
The Guide To Couch Sets For Sale In 2023 sofa warehouse Sale
The 10 Most Terrifying Things About Beans To Coffee Machine Beans to coffee machine
10 Best Mobile Apps For Double Glazing Misting Repair upvc repairs near me (Gary)
The arguments were as compelling as The online persona. I’m totally sold—and not just on The ideas.
15 Startling Facts About Online Shopping Sites List For Clothes You’ve Never Heard
Of Pet Bed For Medium Breeds
7 Tips To Make The Greatest Use Of Your Car Accident Claim Vimeo.com
See What Sectional Sofa Set Tricks The Celebs Are Using modern sectional Couch
10 Tell-Tale Warning Signs You Should Know To Know Before You Buy Door Fitter Leeds upvc door lock repair leeds (Saundra)
Looking Into The Future What Is The Replace Window Glass Near Me Industry Look Like In 10 Years?
Window Replacement
15 Best Pinterest Boards To Pin On All Time About Bmw Key
No Spare
11 Strategies To Completely Redesign Your Local SEO Agencies Local Seo Services
Why Semi Truck Compensation May Be More Dangerous Than You Believed semi truck accident Attorney
[url=https://predniso.online/]prednisone medication[/url]
10 Things You’ve Learned In Preschool That’ll Help You With Best Case CSGO chroma 3 Case
Incontestable Evidence That You Need Sofa Sets For Sale black Leather couch
10 Easy Steps To Start The Business Of Your Dream Car Diagnostic Near Me Business Car Diagnostic Near Me
sobRTZPFLjUAD
How Coffee Bean Coffee Machine Is A Secret Life Secret
Life Of Coffee Bean Coffee Machine Coffee Bean Machines
[url=https://synthroidotp.online/]synthroid 25 mcg coupon[/url]
20 Best Tweets Of All Time Concerning Designer Bags affordable designer Handbags uk
20 Fun Details About Slot Challenges entertaining Slots
The Best Way To Explain Under Desk Treadmill Uk To Your Boss walk Pad For desk
Guide To L30 Dreame: The Intermediate Guide In L30 Dreame L30 Dreame
What’s The Current Job Market For Double Glazed Window Repairs Professionals Like?
double glazed window repairs
The Secret Secrets Of Slot Demo akun demo slot maxwin
Guide To Double Glazing Near Me: The Intermediate Guide
In Double Glazing Near Me double Glazing near me
What’s The Current Job Market For Best Dildos For Squirting
Professionals? best dildos for squirting – Drusilla –
What’s The Reason You’re Failing At Replacement Handles For Upvc Windows domestic
Why No One Cares About Veleco Uk affordable Mobility scooter
Why People Don’t Care About Autowatch Ghost Installers Birmingham ghost Installer
[url=http://lisinoprilgp.online/]3 lisinopril[/url]
Five Killer Quora Answers To Walking Pad For Standing Desk walking pad for standing
desk – Tamika –
Unexpected Business Strategies That Helped
Birth Injury Lawyers To Succeed Darby Birth Injury Law Firm (Vimeo.Com)
15 Autowatch Ghost Installation Benefits Everybody Must Be Able To bmw x5 ghost installer
What’s The Current Job Market For Window.Replacement
Near Me Professionals? window.Replacement Near me
You’ll Never Be Able To Figure Out This Replacement Window Handle’s Secrets replacement
window handle (en.easypanme.com)
10 Facts About Double Glazed Window Suppliers Near Me That Can Instantly Put
You In A Good Mood double Glazing quotes
Is Marc Jacobs Tote Bag Leather The Best Thing There Ever Was?
Marc jacobs medium Tote bag
20 Trailblazers Are Leading The Way In Veterans Disability Attorney lawsuits
The 3 Most Significant Disasters In Self Emptying Robot Vacuum And Mop
The Self Emptying Robot Vacuum And Mop’s 3 Biggest Disasters In History robot vacuum
mop combo self empty (Karolyn)
Five Killer Quora Answers To Treadmills UK Reviews
Treadmills Uk Reviews
Ten Things You’ve Learned In Kindergarden Which
Will Aid You In Obtaining Shopping Online Site Clothes Men’s Mountain Biking Helmet
20 Myths About Locksmiths For Cars Near Me:
Busted locksmith car key replacement (Lora)
14 Businesses Are Doing A Fantastic Job At Double.Glazing Near Me replace double Glazed window
Three Reasons To Identify Why Your Link Login Gotogel Isn’t Working (And How To Fix It) Go Togel
The 10 Most Terrifying Things About Leeds Double Glazing Leeds double glazing
Prada Black Handbags: It’s Not As Expensive As You Think prada Handbags pink
The 10 Most Scariest Things About Waitrose Groceries Online Shopping Uk waitrose
groceries online shopping uk; Kelsey,
Guide To Locked Out Of My Car Help: The Intermediate Guide To Locked
Out Of My Car Help locked out of my car help
13 Things You Should Know About Auto Lock Smith That You Might
Not Have Known 24 hour auto locksmith Near me
The Top Reasons People Succeed Within The Robot Vacuum For Pet Hair Industry Top
[url=https://bestmedsx.com/]american pharmacy[/url]
You’ll Never Guess This Window Replacement Companies’s Tricks Window replacement companies (fpcom.co.kr)
10 Situations When You’ll Need To Know About Double Memory Foam Mattresses mattress Double price
You’ll Never Guess This Link Alternatif Gotogel’s
Tricks link alternatif gotogel (159.89.193.186)
10 No-Fuss Methods For Figuring Out Your Autowatch
Ghost Installers Midlands autowatch ghost Installers
11 Ways To Fully Defy Your Old Avon Perfumes old avon perfumes (Howard)
10 Wrong Answers To Common Window Repairs Luton Questions Do You Know The Right
Ones? aluminium windows luton (https://b.cari.com.my/home.php?mod=space&uid=2920703&do=profile)
5 Killer Quora Answers On Link Login Gotogel link login gotogel [Ola]
How To Beat Your Boss On Online Shopping Uk For Clothes Matte Black Wall Decor Frame
5 Killer Quora Answers On Double Glazed Window Repair window repair
20 Resources That Will Make You Better At Best Self Emptying Robot Vacuum Bob Robot Vacuum (Rossoneri.Ge)
A Productive Rant About Coffee Machine Sage Office Coffee Machines
The Most Pervasive Problems In Locksmith For Cars Near Me Lock smith for cars
(moparwiki.win)
The Best Mesothelioma Law Techniques To Make A Difference In Your Life eunice mesothelioma attorney
You’ve presented a hard to understand topic in a clear and engaging way. Bravo!
5 Killer Quora Answers On Leather Couches For Sale leather couches for sale (isaevclub.ru)
Who Is Responsible For A Akun Demo Slot Budget?
12 Tips On How To Spend Your Money Slot Demo Rtp
Guide To Self Emptying Vacuum: The Intermediate Guide For Self Emptying Vacuum Self emptying Vacuum
A Trip Back In Time What People Talked About Black Friday Robot Vacuum Deals
20 Years Ago best inexpensive robot vacuum
15 Best Fiat 500 Key Replacement Bloggers You Must Follow fiat punto Locked Keys in car
The Porn Star Kayleigh Wanless Success Story You’ll Never Imagine Porn Stars
The 10 Most Scariest Things About Beco Washing Machine beco washing machine (https://images.google.cm/Url?sa=t&url=https://hikvisiondb.webcam/wiki/20_Myths_About_BekoWashing_Machine_Busted)
7 Tips About Mobile Diagnostic That Nobody Will Tell
You engine diagnostic
You’ll Never Be Able To Figure Out This Top British Pornstars’s Secrets Top British Pornstars
What Is The Future Of Tumble Dryers Heat Pump Vs Condenser Be Like In 100 Years?
CandyMama 9KG Heat Pump Tumble Dryer – Fast Drying (https://www.washersanddryers.co.uk/products/9kg-heat-pump-condenser-tumble-dryer-candymama)
This Is The Advanced Guide To CSGO Case Battle case Gamma
10 Car Accident Lawyer Tips All Experts Recommend wadsworth car accident law firm
5 Laws That Anyone Working In Michael Kors Handbags On Sale Should Be Aware Of
michael kors handbag pink [Leila]
How Birth Injury Lawyers Can Be Your Next Big Obsession south river birth injury
law firm; https://vimeo.com/707272106,
Why All The Fuss About How To Ship To Ireland From Uk?
vimeo.Com
You’ll Never Guess This Replacement Windows Near Me’s Secrets
replacement Windows Near me
10 Real Reasons People Dislike Comfortable Couches For Sale Comfortable
Couches For Sale most comfortable Couch
See What Car Key Replacement Ford Tricks The Celebs Are Using key replacement ford
A Brief History Of Text Rewriter In 10 Milestones ai rewriter
Why People Don’t Care About Upvc Doors Leeds Double glazing repair
Why Is Mobile Diagnostics So Effective During COVID-19 How Much Is Car Diagnostic Test
For Whom Is Treadmill For Sale And Why You Should Consider Treadmill
For Sale Treadmills For Sale Near Me
Guide To Black Friday Sofa Sale: The Intermediate Guide In Black Friday
Sofa Sale black friday sofa sale
Guide To Double Glazed Window Replacement: The Intermediate Guide The Steps To Double Glazed Window Replacement double Glazed
window replacement (http://cf58051.tmweb.Ru/)
20 Tips To Help You Be More Effective At Automatic Vacuum
And Mop Robot self Cleaning robot vacuum
25 Surprising Facts About Couches On Sale modern Couch Set
What’s The Current Job Market For Double Glazing Repairs Near Me Professionals Like?
double glazing repairs near me (rentry.co)
See What Designer Handbags On Sale Tricks The Celebs Are Using designer handbags on sale – jisuzimu.com
–
The Reasons Why What CSGO Cases To Invest In Is Everyone’s Obsession In 2023 huntsman weapon case
See What Nespresso Vertuo Pop Machine Tricks The Celebs Are Using nespresso vertuo pop machine, Maximilian,
Designer Handbags To Sell Tools To Improve Your Daily Life Designer
Handbags To Sell Trick That Should Be Used By Everyone Know Bag
Is Audi Replacement Key Cost As Important As Everyone Says?
Audi A1 keys
Guide To Tiktokpornstar: The Intermediate Guide In Tiktokpornstar tiktokpornstar
Find Out What Best Robot Vacuum The Celebs Are Making Use Of small robot vacuum
It’s A L Shaped Couches For Sale Success Story You’ll Never Be Able To 3 seater couch For sale
10 Steps To Begin Your Own Veterans Disability Case
Business veterans Disability lawyers
The Most Prevalent Issues In Injury Compensation Jackson Injury Attorney
10 Meetups About Fiat 500 Keys You Should Attend fiat 500 Duplicate Key
What Is The Reason Slot Demo Gratis Is Right For You play Demo
Why Side By Side Fridge And Freezer Is The Right Choice For You?
Freezer and cooler
10 Beautiful Graphics About Locksmith Car Key car keys locksmith
IyMxbAPrciBKFV
We’ve Had Enough! 15 Things About Saab Key Fob Programming We’re Sick Of Hearing how to program saab 9-3 key fob
10 Quick Tips About Auto Accident Claim vimeo.Com
You’ll Be Unable To Guess Shopping Online Uk To Ireland’s Secrets shopping online uk
Door Fitters Leeds It’s Not As Hard As You Think Repair double glazing
8 Tips To Improve Your CSGO Cases Opening Game web case
How Can A Weekly Replacement Double Glazed Glass Only Near Me Project
Can Change Your Life double glazing Windows handles
What’s The Current Job Market For Online Shopping Clothes Uk Cheap Professionals?
online shopping clothes uk Cheap
10 Automotive Locksmith Tricks All Experts Recommend Mobile Auto Locksmiths Near Me
You’ll Never Be Able To Figure Out This Double Glazing Repair Leeds’s Benefits
Double glazing repair
Misted Double Glazing Repairs Tools To Streamline Your Everyday Lifethe Only Misted Double Glazing
Repairs Trick That Everyone Should Learn Double glazing repairs
Guide To Porn Star Kayleigh Wanless: The Intermediate Guide In Porn Star Kayleigh Wanless porn Star Kayleigh wanless (vesti46.Ru)
You’ll Never Guess This Online Shopping Sites Top 7’s Benefits Online Shopping Sites Top 7
How Window Replacement Near Me Became The Hottest Trend Of 2023 wooden
You’ll Never Be Able To Figure Out This Double Glazed Units Near Me’s Secrets double Glazed units near Me
There Is No Doubt That You Require Birth Defect Litigation birth defect law
firm – Magaret –
Under Desk Treadmill: What’s New? No One Has Discussed Compact Ultra Slim Treadmill For Home/Office Fitness
Could Bmw Key Replacement Be The Key To 2023’s Resolving?
Replacement Bmw Key
Why We Are In Love With Marc Jacobs Crossbody Bag (And You Should Also!) Marc jacobs handbags Black
10 Things You Learned In Kindergarden Which Will Aid You In Obtaining Bentley Continental Gt Key Fob bentley flying Spur Key
Guide To Kayleigh Wanless Pornstar: The Intermediate Guide Towards
Kayleigh Wanless Pornstar kayleigh wanless pornstar
The Reason Why Joker123 Gaming Is Everyone’s Desire In 2023 spam
Private ADHD Titration Tools To Make Your Daily Life Private
ADHD Titration Trick That Should Be Used By Everyone Know private adhd titration
15 Pinterest Boards That Are The Best Of All Time About Green Power green scooter
10 Things That Everyone Doesn’t Get Right About Programing Car Keys Locksmith Near Me Program Key Fob
Guide To Uk Online Shopping Sites Like Amazon: The Intermediate Guide The Steps
To Uk Online Shopping Sites Like Amazon uk online shopping
sites like amazon – Chassidy –
The One Replacement Ford Fiesta Key Uk Mistake That
Every Newbie Makes Ford transit Keys
The One Car Key Programmer Near Me Trick Every Person Should Know Car Key Programming Cost
Are You Responsible For An Upvc Window Repairs Budget?
10 Unfortunate Ways To Spend Your Money upvc Window repairs near me
How To Get More Benefits Out Of Your Double
Glazing Near Me fit
A Provocative Rant About Tommy Hilfiger Xbody Bag Ladies Bag Tommy Hilfiger
7 Simple Strategies To Completely Rolling With Your Mesothelioma Settlement Absecon Mesothelioma Lawsuit
What Is What CSGO Cases To Invest In And Why
Is Everyone Talking About It? Cs20 Case
You’ll Be Unable To Guess Double Glazed Units Near Me’s Tricks Double Glazed Units Near Me
20 Resources To Help You Become More Efficient With Accident
Attorney Near Me utah accident attorney, smart-watchs.ru,
The 10 Most Scariest Things About Upvc Window Repairs upvc window repairs near me (http://www.Cskfloor.com)
What’s The Current Job Market For Double Glazing Door Repairs Professionals?
Door repair
Bed Settee For Sale Tools To Ease Your Everyday Lifethe Only Bed Settee For Sale Trick That Every Person Should Know Bed Settee For Sale
The 10 Most Scariest Things About Best Vibrators For Clit best vibrators for clit (Adell)
5 Vauxhall Key Replacement Lessons From The Pros vauxhall Insignia key fob
[url=http://happyfamilystorerx.online/]top online pharmacy 247[/url]
You’ll Be Unable To Guess Dreametech L30’s Benefits dreametech l30 (security-hub.Com.ua)
What Average Cost Of Car Diagnostic Uk Experts Want You To Know diagnostics
How To Save Money On Secondary Double Glazing Near Me Secondary Double Glazing
9 Lessons Your Parents Taught You About Washing Machine 12kg Price washing machine 12kg Price
Why Is Double Glazing Repair Leeds So Popular? broken
5 Laws Anybody Working In Repair Timber Windows Should Be Aware Of wooden
What’s The Job Market For Double Glazing Door Repairs Near Me Professionals?
door Repair
10 Replacement Key Bmw That Are Unexpected Lost Bmw Keys
Are You Responsible For A Double Glazed Window Repair Budget?
12 Best Ways To Spend Your Money upvc Door repairs near me
11 Methods To Totally Defeat Your Affordable SEO Agency affordable seo Services for small Businesses
11 Ways To Destroy Your Double Ended Dildo double-ended dildos
10 Tell-Tale Warning Signs You Need To Get A New Glimmerstick Eyeliner glimmerstick eyeliner blackest black
10 Failing Answers To Common Beko Washing Machine Questions:
Do You Know The Correct Ones? beko washing machine buy
Can Upvc Window Repairs Ever Be The King Of The World?
Window repairs Near me
The People Who Are Closest To Window Repair Near Me Have Big Secrets To Share upvc door repairs near me
(Kisha)
How Amazon Uk Online Shopping Clothes Has Become The Top Trend On Social Media vimeo
20 Things You Need To Know About Shopping Online Uk Clothes
online shop
The Top 5 Reasons People Thrive In The CS GO New Cases Industry Cs20 Case
20 Fun Facts About Online Shop 50X60 Inch Throw Blanket
5 Laws That Anyone Working In Railroad Injuries Compensation Should Know railroad injury attorneys –
Duane,
10 Things That Your Family Teach You About Double Glazed Window Suppliers Near Me Double Glazed Window Suppliers Near Me
The 10 Most Scariest Things About Asbestos Claim asbestos Claim
Are You Responsible For The Railroad Injuries Compensation Budget?
12 Top Ways To Spend Your Money fort walton beach railroad injuries law firm
The One Rabbit Vibrator Uk Trick Every Person Should Learn bunny vibrators –
https://funsilo.date/wiki/15_Reasons_why_you_shouldnt_be_ignoring_cheapest_rabbit_vibrators –
What Is Everyone Talking About Truck Accident Lawyer Right Now hallandale beach truck accident attorney
A The Complete Guide To Dream L30 Ultra From Start To Finish dreame l30 vs l20 (telegra.ph)
Is Auto Fold Mobility Scooter The Greatest Thing There Ever Was?
Best auto folding mobility scooter uk
The Reasons Birth Injury Lawyers Is Everywhere This Year Vimeo.com
The 9 Things Your Parents Teach You About Replace Window Handles Replace window handle
The One Slot Demo Pragmatic Mistake That Every Newbie Makes akun Demo
terlengkap (Mco21.ru)
10 Quick Tips About Bean To Cup Coffee Machine Sale http://Www.Coffeee.Uk
10 Quick Tips For Double Glazing Unit Repair window replacement near Me (surgical-instruments.tmsmed.net)
What’s The Job Market For Veleco Mobility Scooters Uk Professionals?
veleco mobility [http://www.google.co.uz]
See What London Double Glazing Tricks The Celebs Are Using london double glazing (Lamar)
What’s The Reason Mercedes Key Fob Is Fastly Changing Into The Hottest Fashion Of 2023 key Mercedes
See What Sectional Sleeper Sofa Tricks The Celebs Are Making Use Of sectional sleeper sofa; https://lee-french.thoughtlanes.net/12-statistics-about-mini-sectional-sofa-to-make-you-look-smart-around-the-cooler-cooler/,
Can Electric Mobility Scooters For Sale Ever Rule The World?
mobility scooter near Me for sale (cs.Xuxingdianzikeji.com)
Хотите окунуться в мир приключений на Байкале? Планируете поездку на Байкал в 2024 году? FanatBaikala.ru готов предложить вам самые выгодные варианты. Посетив их сайт, вы сможете узнать [url=https://fanatbaikala.ru/tours]поездка на Байкал стоимость 2024[/url] и выбрать наиболее подходящий для вас тур.
FanatBaikala.ru предлагает разнообразные варианты отдыха на Байкале по самым привлекательным ценам. От путешествий на комфортабельных катерах до увлекательных экскурсий по окрестностям – здесь вы найдете все необходимое для незабываемого отдыха.
Поездка на Байкал – это не только возможность насладиться красотами природы, но и погрузиться в атмосферу удивительного места. И выбрав [url=https://fanatbaikala.ru/tours]поездку на Байкал цена[/url] которой приемлема для вас, вы сможете в полной мере насладиться этим незабываемым опытом.
Double Glazed Window Repairs: The Good, The Bad, And The Ugly double Glazing repairs
See What Bentley Flying Spur Key Tricks The Celebs Are
Using bentley flying spur key (m1bar.com)
15 Interesting Hobbies That Will Make You Smarter At Under Desk Treadmill With Incline foldable under desk treadmill [Brian]
Mesothelioma Attorney Tools To Improve Your Daily Life Mesothelioma Attorney Trick That Everybody Should Know mesothelioma Attorney
The Best Sectional Couches For Sale Tricks
To Make A Difference In Your Life best sectional Couches
15 Reasons To Not Overlook Folding Scooter mymobilityscooters.Uk
5 Things That Everyone Doesn’t Know On The Subject Of Audi A4
Key Replacement replace audi key
7 Things About Sofas Sale You’ll Kick Yourself For Not Knowing Light grey Couch
Sofas Sectional Tips To Relax Your Daily Lifethe One Sofas Sectional Technique Every
Person Needs To Be Able To Sofas sectional
This Is How Ford Focus Replacement Key Cost Uk Will Look In 10 Years Ford kuga key Replacement
20 Things You Need To Be Educated About Avon Skin So Soft Where To Buy avon skin So soft where to buy
20 Trailblazers Lead The Way In Which CS GO Case Is Best To Open Shadow
Case (Elibrary.Mel.Org)
12 Companies Are Leading The Way In Treadmills Treadmills at home
Are You Responsible For The Upvc Window Repairs Budget? 10 Very Bad Ways To
Invest Your Money window repairs near me (te.Legra.ph)
Why Accident And Injury Attorneys Isn’t As Easy As You
Think bills
15 Things You Don’t Know About Accident Attorneys Utah Accident Attorney (Www2.Americangirl.Com)
10 Top Facebook Pages Of All Time About Self-Emptying
Robot Vacuum For Pets Best self-Emptying robot vacuum for pet hair
15 Weird Hobbies That’ll Make You More Successful At Fridge Freezer what fridge freezer is best
Guide To Double Glazing Window Repairs: The Intermediate Guide For Double Glazing Window Repairs
Double glazing
Situs Alternatif Gotogel Tools To Improve Your Daily Life
Situs Alternatif Gotogel Trick That Every Person Should Know situs alternatif
gotogel (tgpdevil.net)
5 Reasons Nissan Micra Key Replacement Is Actually A
Good Thing programmers
The People Who Are Closest To Butt Plugs Have Big Secrets To Share app controlled Buttplug
Who Is Sex Machines And Why You Should Take A Look online Store
The People Nearest To Bentley Key Share Some Big Secrets nearest
15 Best Pinterest Boards Of All Time About CS GO Battle Case open Cs2
See What L30 Dreame Tricks The Celebs Are Making Use Of L30 Dreame
Ten Things You Shouldn’t Post On Twitter Double Glazing Door
5 Killer Quora Answers On Beko Washing Machine New Beko Washing Machine New
9 Lessons Your Parents Teach You About Upvc Window Repairs window repairs (eng.Worthword.com)
What You Should Be Focusing On Enhancing Replacement Windows Leeds patio door repair Leeds
The 3 Most Significant Disasters In CS GO Gambling Sites The CS GO Gambling Sites’s 3 Biggest Disasters In History case Horizon
Is Technology Making Double Glazed Near Me Better Or Worse?
double glasing
9 Lessons Your Parents Teach You About Spare Car Key Cost
spare car key cost
A The Complete Guide To Local Accident Attorney From Beginning To End accident attorney
bridgeport (https://www.ccasy.com/go/?url=https://www.accidentinjurylawyers.claims/accident-attorneys-near-me)
11 Ways To Completely Redesign Your Replacement Key For Peugeot Partner Van Repairer
10 Things You Learned In Kindergarden Which Will
Aid You In Obtaining 9 Kg Washing Machine 9 Kg washing machines
Check Out: How Auto Accident Compensation Is Taking Over And What You Can Do
About It copperas cove auto accident Lawyer
17 Signs To Know You Work With Glass Window Repair Window Replacement
Side By Side Freezers Fridge Tools To Ease Your Daily Life Side By Side
Freezers Fridge Trick Every Individual Should Learn side by side freezer
You’ll Never Guess This Which Online Stores Ship Internationally’s Tricks which online Stores ship internationally
The 10 Most Terrifying Things About Window Repairs Near Me
window repairs Near Me
It Is The History Of Treadmill For Home In 10 Milestones Treadmill
For Home Use – M.Svr2.Yedam.Com,
9 Signs That You’re An Expert Michael Kors Pink Handbag Expert michael kors Bags On Sale
5 Women Double Dildo Projects For Every Budget TOPS Adult Toys
A Step-By-Step Guide To BMW Battery Replacement Key From Beginning
To End bmw series 1 key
Five Killer Quora Answers To Best Self Emptying Robot Vacuum Self Emptying robot Vacuum
What’s The Ugly Reality About Upvc Window Repairs upvc window repairs near me [Kazuko]
The Cleaning Robot Mop And Vacuum Case Study You’ll Never Forget best robot vacuum for carpet and hardwood
Five Reasons To Join An Online Window Repair Near
Business And 5 Reasons Not To upvc repair
You’ll Be Unable To Guess Gotogel Link Alternatif’s
Tricks gotogel link alternatif
9 Things Your Parents Teach You About Upvc Window Repair Near
Me window repair near me; Trudi,
Seat Car Key Cover 101:“The Complete“ Guide For Beginners seat car keys
One Of The Biggest Mistakes That People Make With Attorney
For Accident Claim accident attorneys houston, Dana,
How Glazing Repairs Near Me Was Able To Become The
No.1 Trend On Social Media double glazing near Me
10 Easy Ways To Figure The CS GO New Cases You’re Looking For operation Broken Fang case
10 Things Everybody Hates About Double Over Double Bunk Bed Two Double Bunk Beds
You’ll Never Be Able To Figure Out This Motorcycle Accident Lawyers’s Tricks Motorcycle Accident Lawyers
The 10 Most Terrifying Things About List Of Online Shopping Sites In Uk list of online shopping sites in uk
11 Creative Ways To Write About Double Glazing Windows Repair Window Repair
The December 2020 final TSCA risk assessment for chrysotile asbestos identified excessive
health risks for humans for all ongoing use
of chrysotile Port orange asbestos law firm.
See What Reallife Sexdolls Tricks The Celebs Are Making Use
Of sexdoll
This article was a joy to read. The enthusiasm is contagious!
10 Ways To Build Your Avon Starter Kit 2023 Empire kits
Guide To Smeg Fridge: The Intermediate Guide To Smeg Fridge smeg fridge, Nate,
The Top Avon Starter Set Experts Have Been Doing 3 Things Avon pack
Why We Enjoy Door Repair Near Me (And You Should,
Too!) Window replacement near me
Five Killer Quora Answers To Window Repairs Near
Me window repairs near me [https://cairns.Nsta.edu.Au/]
What Do You Think? Heck What Is Windows Leeds? Upvc door repair Leeds
Asbestos Compensation Tips That Will Change Your Life asbestos Litigation
20 Amazing Quotes About Pvc Window Repairs double glazing (Chasity)
Five Killer Quora Answers On Washing Machine 12kg Uk
Washing Machine 12Kg Uk (Msichat.De)
Ten Things You Need To Be Aware Of Double Glazing Fitters Near
Me Double glazing window handles
What’s The Job Market For Sofas Sectionals Professionals?
Sofas sectionals
What Is Sectionals For Sale Near Me And Why Is Everyone
Talking About It? big sectional couch
Looking For Inspiration? Try Looking Up Which Online Stores Ship Internationally Bright Toy Kitchen For Kids
You’ll Never Guess This Comfy Couches For Sale’s Tricks comfy couch (wayranks.com)
What Fiat Key Cover Experts Want You To Know? Spare fiat 500 key
Speak „Yes“ To These 5 Evening Work From Home Jobs Tips remote Work from home Jobs
(baoliaotai.cn)
See What Kayleigh Porn Star Tricks The Celebs Are Using kayleigh Porn star
The 9 Things Your Parents Teach You About Double Glazing Window Repairs Near Me Window repairs Near me
Why Nobody Cares About Mesothelioma Attorney porter mesothelioma lawyer
The Reason Why Bentley Continental Key Is Everyone’s Desire In 2023 bently car key
– Connie –
Are You Confident About Doing Seat Key? Check This Quiz seat ibiza Keys
Why No One Cares About Mobility Scooters For Sale Near Me Mobility Scooters online
How To Save Money On Car Key Locksmith Near Me mobile locksmith near me for cars (https://have-torres.federatedjournals.com/)
See What Washing Machines 12kg Tricks The Celebs Are Using Washing machines 12kg
10 Graphics Inspirational About Double Glazing Near Me pvc
7 Essential Tips For Making The Most Of Your Pornstar Porn Star
What’s Holding Back What’s Holding Back The Best Online Shopping Websites Uk Industry?
vimeo.com
20 Double Glazing Repairers Websites Taking The Internet By
Storm upvc window Repairs
An Intermediate Guide Towards Upvc Windows Repair upvc window Repair (fpcom.co.Kr)
The 10 Scariest Things About Online Shopping Top 7 online shopping top 7 (http://lamdongtv.vn)
11 Ways To Fully Redesign Your Accident Attorney Near Me accident attorney lake charles, http://www.google.com/url?q=https://download.programmer-books.com/?link=https://www.Accidentinjurylawyers.claims/Accident-attorneys-near-me/,
The 10 Scariest Things About Leeds Double Glazing Leeds double glazing
15 Interesting Facts About Dangerous Drugs Attorney That You’d Never Been Educated About Vimeo
11 Ways To Fully Redesign Your Double Glazed Window Repair double glazing repair (https://www.derhonig.de/firmeneintrag-bearbeiten?nid=710&element=http://dnpaint.co.kr/bbs/board.php?bo_table=B31&wr_id=4171203)
15 Bizarre Hobbies That’ll Make You More Effective At Asbestos Law Asbestos compensation
Why No One Cares About Best Accident Attorney queens accident attorney – http://Www.archeus.ro –
You’ll Never Guess This 4 Wheel Mobility Scooters’s
Secrets 4 Wheel Mobility
„Ask Me Anything“: Ten Responses To Your Questions
About 9kg Capacity Washing Machine 9kg capacity washing Machines
Why You Should Be Working With This Robot Vacuum
Cleaner With Mop Best Robot vacuum mop
How Bags For Women Tommy Hilfiger Has Become The Top Trend
On Social Media bag tommy Hilfiger sale
Coffee Machine With Pods Explained In Fewer Than 140 Characters top
rated coffee machines (https://Storescripts.ru/user/verseskate98/)
See What Treadmill Home Gym Tricks The Celebs Are Using Treadmill home gym
What Is Semi Truck Lawyer? History Of Semi Truck Lawyer In 10 Milestones Semi Truck Accident Lawyer [54.Staikudrik.Com]
30 Inspirational Quotes For Online Shopping
Uk Cheap Small Pet Home On Casters
Three Of The Biggest Catastrophes In Local Locksmith For Cars The
Local Locksmith For Cars’s 3 Biggest Disasters In History
key
Treadmill Under Desk Is The Next Hot Thing In Treadmill Under Desk Walking treadmill
under Desk (Fiaipmanager.fiaip.it)
5 Killer Quora Answers On Sofas Sale chaise
What’s The Current Job Market For Auto Door Lock Repair Near Me Professionals?
auto Door lock Repair Near me
Don’t Make This Silly Mistake On Your Repair
Double Glazing Window doctor
This Story Behind Semi Truck Case Will Haunt You For The
Rest Of Your Life! Lawsuit
12 Facts About Double Glazing Window Repairs That Will Make You Think Twice About The Cooler
Water Cooler upvc windows repair near me (Rhonda)
See What Online Shopping Figures Uk Tricks The Celebs Are
Using online shopping figures uk (https://maps.google.Co.ug/)
The People Who Are Closest To Window Replacement Near
Me Share Some Big Secrets window replacement Companies
15 Amazing Facts About Birth Injury Lawyers That You Never Knew birth injury lawsuit
17 Signs To Know If You Work With Espresso Machines Office
What Is Best Online Shopping Uk Clothes And Why Is Everyone Dissing It?
Energy-Efficient Light Bulbs
See What Affordable SEO Firm Near Me Tricks The Celebs Are Using
affordable seo firm near me (Soila)
See What Amazon Uk Online Shopping Clothes Tricks The Celebs Are Using amazon uk online Shopping clothes
See What Best Rated Robot Vacuum Tricks The Celebs Are
Making Use Of best rated robot vacuum
The 10 Most Terrifying Things About Metal Anal Butt Plugs plug
What Double Bunk Experts Want You To Be Educated Double over double bunk beds
An asbestos attorney with experience can help you obtain the justice you deserve.
A lawyer with these qualifications can help you
file a trust claim or lawsuit for mesothelioma.
My website … vimeo
What Is The Reason Volkswagen Key Replacement Cost Is The Best Choice For You?
price
A Look At The Ugly Reality About Couches On Sale small l shaped couch
(https://fakenews.win/wiki/15_Terms_That_Everyone_Is_In_The_Couches_L_Shape_Industry_Should_Know)
What Mesothelioma Settlement Is Your Next Big Obsession? middleton Mesothelioma lawyer;
Vimeo.com,
The Trucking Lawyers Awards: The Most Sexiest, Worst, And The Most Unlikely Things We’ve Seen Vimeo.Com
Find Out What CSGO Case Battle Tricks Celebs Are
Using cases
A Brief History Of Backlink Building Software History
Of Backlink Building Software seo
There Are A Few Reasons That People Can Succeed In The Accident Law Industry accident lawsuit; clients1.google.fr,
Five Killer Quora Answers On British Porn Stars
Xxx british porn Stars xxx
Ten Double Glazed Replacement Glass Near Me That Will Actually Improve Your Life double glazing window repairs – Matthew –
10 Places Where You Can Find Find Accident Attorney deserve
Guide To Upvc Repairs Near Me: The Intermediate Guide Towards
Upvc Repairs Near Me upvc Repairs Near me (p3Terx.com)
Why The Poker Online Is Beneficial In COVID-19 goblok
Self Empty Robot Vacuum And Mop Techniques To Simplify Your Daily Life Self Empty Robot
Vacuum And Mop Trick That Every Person Must Learn self empty robot Vacuum and mop
10 Myths Your Boss Has Regarding Kids Bunk Beds Buy bunk bed
How To Find The Perfect Mesothelioma Claim On The Internet mesothelioma legal advice
Five Things You Didn’t Know About Under Counter Fridge fridge glass
door (Indira)
How To Become A Prosperous Double Glazed Replacement Glass
Near Me Even If You’re Not Business-Savvy upvc double glazed windows
10 Facts About Male Anal Sex Toy That Will Instantly Put You In The
Best Mood toys for Anal
The No. 1 Question Anyone Working In Cerebral Palsy Litigation Should Know How To Answer Williston cerebral palsy attorney
(vimeo.com)
What’s The Job Market For Double Glazed Window Repairs Professionals Like?
Double Glazed Window Repairs
Replacement Audi Key Techniques To Simplify Your Daily Lifethe One Replacement Audi Key Trick
That Should Be Used By Everyone Be Able To replacement Audi key
10-Pinterest Accounts You Should Follow Accident Attorney Lawyer florida accident Attorney
10 Locations Where You Can Find Online Shopping
Uk Sites Electric Torque Wrench
Erb’s Palsy Settlement: The Secret Life Of Erb’s Palsy Settlement erb’s palsy lawsuit (Ola)
Responsible For The Akun Demo Slot Budget? 10 Unfortunate Ways To Spend Your Money
demo slot tergacor – bitsch-hay-2.hubstack.net,
Looking Into The Future What’s In The Pipeline? Robot Vacuum And Mop With Self
Empty Industry Look Like In 10 Years? robot vacuum Mop combo Self empty
The 10 Scariest Things About Walking Pad And Standing Desk walking pad and standing desk (Amelie)
Ten Injury Case Myths That Aren’t Always True Greensburg Injury Law Firm
Where Will Repairing Upvc Windows One Year From In The Near Future?
near
Guide To Best Price 12kg Washing Machine: The Intermediate Guide To Best Price 12kg Washing Machine Best Price 12Kg washing machine
„Ask Me Anything:10 Responses To Your Questions About Lg American Fridge Freezer samsung american fridge freezer with water and ice dispenser (Dianna)
10 Beautiful Graphics About Personal Injury Attorneys broomfield personal Injury Lawyer
15 Top Pornstars Benefits You Should All Be Able To
photos
Five Killer Quora Answers On Cheap Butt Plugs cheap butt plugs
What’s The Current Job Market For Double Glazing Door
Repairs Near Me Professionals Like? Door repair
Are Best CSGO Case To Open The Most Effective Thing That Ever Was?
horizon Case
Pay Attention: Watch Out For How Upvc Doors Leeds Is Taking Over And What We
Can Do About It upvc window Handle repair leeds
5 Killer Quora Answers On Repair Misted Double
Glazing Near Me double glazing Near Me
The 10 Scariest Things About Gotogel Link Alternatif link Alternatif gotogel
What’s The Job Market For Uk Online Phone Shopping Sites Professionals?
uk online phone shopping sites (http://Www.google.com/url?q=https://Vimeo.com/932264176)
Top Accident And Personal Injury Lawyers Explained
In Fewer Than 140 Characters Tampa Personal Injury Lawyer
The 9 Things Your Parents Teach You About Double Glazing Repair Near Me double glazing Repair near me
How To Tell If You’re Set To Go After Double Glazing Repair Near Me double glazing Window handles
The Reasons Misted Double Glazing Repairs Is Everywhere This
Year Window Replacement Near Me
The 10 Most Scariest Things About List Of Online Shopping Sites In Uk
list of online Shopping sites in uk; http://gorodaleksandrov.ru/,
Guide To Self Emptying Robot Vacuum For Pets: The Intermediate Guide On Self Emptying Robot Vacuum For Pets Self emptying robot vacuum for pets
What’s The Job Market For Double Glazed Repairs
Near Me Professionals Like? Double Glazed Repairs Near Me
Seven Explanations On Why Autowatch Ghost Installers Is So Important
Installing Ghost Immobiliser
What’s The Current Job Market For Window Doctor Near
Me Professionals Like? window doctor near me (Dorine)
The 10 Most Scariest Things About Bunk Bed Kids bunk
bed kids; Novella,
[url=https://ezithromycin.online/]azithromycin prices[/url]
It’s The One Can I Buy From A Uk Website Trick Every Person Should
Learn small Area Rug
Where Do You Think Robot Vacuum Be 1 Year From
Today? cheap robot vacuum cleaner – Rodolfo –
16 Must-Follow Facebook Pages For Audi Keys
Replacement Marketers audi key fob replacement (Vicky)
10 Misconceptions Your Boss Shares About Luton Window Repair Luton Window
Repair Glass Specialists Near Me
Get To Know With The Steve Jobs Of The Buy Anal Sex Toys Industry anal toys shop
Washing Machines Beko Tools To Improve Your Daily Life Washing
Machines Beko Trick That Every Person Should Know washing Machines beko
From Around The Web Twenty Amazing Infographics About Robotic
Shark Vacuum Best robot vacuum for dog hair
The Reasons Railroad Injuries Case Is Fast Becoming The Hottest Fashion Of 2023 Railroad Accidents
The 10 Most Scariest Things About Replacement Window Glass Replacement Window Glass
Five Killer Quora Answers On Adultwork Pornstar adultwork Pornstar
Joker123 Gaming: What’s The Only Thing Nobody Is Talking About goblok
What’s The Current Job Market For Link Daftar Gotogel Professionals?
link daftar Gotogel
12 Companies Leading The Way In Car Key Replacement Audi Service
See What Bentley Arnage Key Fob Tricks The Celebs Are Making Use Of bentley arnage key fob (Leonel)
15 Funny People Working In 2nd Hand Mobility Scooters Near Me In 2nd Hand Mobility
Scooters Near Me mobility Aids
Why Will CSGO Case Prices Go Down Is More Risky Than You Thought
case fracture – Leola –
What Are The Myths And Facts Behind Fiat 500 Spare Key Fiat 500 car keys
The 10 Most Scariest Things About Shopping Online Uk Which Supermarket Is Cheapest For Online Shopping
(Buehnehollenthon.At)
The 9 Things Your Parents Taught You About Double Glazed Windows Near Me double glazed windows near me
17 Signs You Work With Double Glazing Companies Near Me double glazing companies near me
Its History Of Locksmith For Cars fobs
10 Instagram Accounts On Pinterest To Follow Veterans
Disability Attorney gardner veterans disability Lawyer
What’s The Current Job Market For Accident Claims Lawyers Professionals?
accident claims lawyers
7 Things About Repairing Double Glazing You’ll
Kick Yourself For Not Knowing Upvc door repairs near me
5 Laws That Can Benefit The Best CSGO Opening Site Industry
counter-strike cases; Lacey,
Futon For Sale’s History History Of Futon For Sale blue couch [Zara]
Beware Of This Common Mistake You’re Using Your Slot Themes themed Slots
Looking For Inspiration? Look Up What Is The Best Online Shopping In Uk Xenon Hid D1S Bulb
The 10 Most Scariest Things About Glass Window Repair window repair
10 Ways To Create Your Franklin Mesothelioma Lawyer Vimeo Empire arizona Mesothelioma lawyers
What You Should Be Focusing On Improving Window Repairman services
9 Things Your Parents Teach You About London Window And
Door London Window And Door
Find Out More About Mens Masturbation Toys While Working From Home male
vibrators (thoughtful-ferret-fnqkkn.Mystrikingly.Com)
pwiPXYAJbWZOzG
15 Top Pinterest Boards From All Time About Asbestos Asbestos Attorney
The Hidden Secrets Of Vibrating Panty Uk Vibrator Orgasm Panties
See What Small American Fridge Freezer Tricks The Celebs Are Making Use Of small American fridge freezer (https://www.seaboardfcu.com)
10 Tell-Tale Symptoms You Must Know To Find A New
Mesothelioma Claim attorney cancer law lawyer mesothelioma (Lauri)
Over the past century asbestos was used to make thousands of items stronger and more
resistant to fire, and cheaper. Exposure to asbestos fibers in the air can lead
to lung disease such as asbestosis or mesothelioma.
Also visit my page: vimeo.com
5 Laws That Anyone Working In Replacement Bmw Key Should Be Aware
Of Lost
The Next Big Thing In The Replacement Car Keys Ford Industry ford fiesta key replacement Cost
15 Gifts For The Locksmiths Near Me For Car Lover In Your Life locksmith for Car key
Ten Ford Key Replacement Near Me That Will Actually Help You Live Better Ford Kuga Key Replacement
A Provocative Rant About Under Counter Fridge fridge For sale near me
14 Businesses Doing A Great Job At 4 Wheel Electric Mobility
Scooter 4 wheel all Terrain Electric scooter; kingranks.Com,
The 9 Things Your Parents Teach You About Double Glazing Window Repairs Near Me window repairs near me (http://Www.Combpm.com/)
7 Useful Tips For Making The Most Out Of Your Avon Start Smart Bag 2023 Avon Starter Kit 2024
What’s The Job Market For Sofa Sets For Sale Professionals?
sofa sets for sale (Kirsten)
Why Pavement Mobility Scooter Is Relevant 2023 pavement Accessibility scooters
10 Life Lessons That We Can Learn From Window Seal Replacement
window replacement Companies
10 Healthy Habits For Examples Of Online Products pneumatic test ball (vimeo.Com)
Guide To Bunk Beds Double: The Intermediate Guide In Bunk Beds
Double bunk beds double (Julia)
Double Glazing Repairs Near Me Tools To Improve Your Daily
Life Double Glazing Repairs Near Me Trick That Every Person Should Know double glazing repairs near me
The No. 1 Question Everyone Working In Bentley Key Fob Programming Needs To
Know How To Answer remote
What’s The Ugly Facts About Sexdoll Realistic female sex
doll (sunangle.co.kr)
What’s The Job Market For Double Glazing Door Repairs Professionals?
door Repair
10 Websites To Help You Become An Expert In Treadmill Under Desk walking Treadmill under desk
You’ll Never Be Able To Figure Out This Lock Smith For Cars’s Benefits
lock smith For cars
Ten Things You Learned At Preschool That’ll Help You Understand Sex Machines
Online Store sex machines uk
How Much Can Audi Car Key Experts Earn? audi car
key replacement (Alisha)
The Reasons Amazon Uk Online Shopping Clothes Is Harder Than You Imagine
Vimeo
Local SEO Expert Near Me Tools To Make Your
Daily Lifethe One Local SEO Expert Near Me Trick That
Everyone Should Learn local Seo Expert near Me
Top 10 Online Shopping Sites In Uk For Clothes Tools To
Help You Manage Your Everyday Lifethe Only Top 10 Online Shopping Sites
In Uk For Clothes Trick That Everyone Should Learn top 10 online shopping Sites in uk for clothes
What’s The Job Market For Top Pornstar Only Fans Professionals Like?
top Pornstar only fans
5 Double Glazing Window Repair Projects For Any Budget nearby
10 Things That Your Family Taught You About Upvc Window Repairs Window Repairs
10 Healthy Habits To Use Treadmills treadmills for sale (Danial)
11 Ways To Fully Defy Your Larder Fridge frydge
The Sage Advice On Self Empty Robot Vacuum From A Five-Year-Old self Empty robot vacuum mop
9 Lessons Your Parents Teach You About L30 Ultra Dreame l30 Ultra Dreame
[url=http://metforminbi.online/]metformin 2000mg daily[/url]
[url=http://bestmedsx.com/]pharmacy com[/url]
Learn More About Treadmill Near Me While Working From
Your Home treadmill for sale (Clients1.google.com.kw)
The Little-Known Benefits Of Mesothelioma Spring grove mesothelioma law Firm
The 10 Most Terrifying Things About Double Glazing Companies Near Me double glazing companies near
me (Nila)
What’s Everyone Talking About Robot Vacuum’s This Moment best inexpensive robot Vacuum
The 10 Most Terrifying Things About Link Alternatif
Gotogel Link alternatif gotogel
10 Things You’ve Learned From Kindergarden To Help You Get Leeds Window Repair double glazing leeds (Beatrice)
See What Online Clothing Sites Uk Tricks The Celebs Are Utilizing Online clothing Sites uk
The 10 Scariest Things About Marc Jacobs Handbags Sale Marc Jacobs Handbags Sale
Why Car Accident Case Is A Must At The Very Least Once In Your Lifetime Vimeo
This Week’s Top Stories About Fleshlight Cleaning Fleshlight Cleaning best Fleshlight insert
What Is Replacement Double Glazed Glass Only Near Me And Why Is
Everyone Talking About It? double glazing window (Melina)
Guide To Online Shopping Uk Discount: The Intermediate Guide On Online Shopping Uk Discount online shopping uk discount (39.cholteth.com)
The 15 Things Your Boss Wished You’d Known About Waitrose Groceries Online Shopping Uk {Wahl Arco Equine Clipper Kit|Cordless Horse Clippers|Professional Equine Grooming Tools|Horse
Cordless Clipper Kit|Animal Clippers For Horses|Animal Grooming Clippers Professional|Wahl (https://Vimeo.com/932422742)
9 . What Your Parents Taught You About Examples Of Online Shopping Examples of online shopping
10 Startups That’ll Change The Window Glass Replacement Industry For
The Better Window Glass Replacement Near Me
This Is The Good And Bad About Online Shopping
Websites Clothes online shopping websites for Clothes
7 Things You’ve Never Learned About Integrated Fridge
Freezer Side By Side what Is The Best side by Side Fridge freezer
You’ll Never Guess This Window Replacement Companies’s Secrets Window replacement companies
See What Toledo Birth Injury Attorneys Tricks The Celebs Are Making Use Of toledo
birth injury attorneys (Maple)
A Proficient Rant Concerning Double Glazed Window Suppliers Near Me double Glazing Quotes
The Railroad Injuries Attorneys Success Story You’ll
Never Believe Railroad Accident Lawyer Near Me
20 Things You Need To Know About Used Couches For Sale sectional couches With recliners
Nine Things That Your Parent Taught You About Beaumont Mesothelioma
Attorney Beaumont Mesothelioma Attorney [Articlement.Com]
Don’t Believe These „Trends“ Concerning Michael Kors Designer Bags Michael Kors Handbags Green
The 10 Scariest Things About Key Repair Near Me key
The Most Inspirational Sources Of Avon Skin So Soft Dry Oil Spray Uses avon skin so Soft dry oil spray uses
15 Top Mesothelioma Legal Question Bloggers You Must Follow hubbard mesothelioma attorney, Raymond,
9 . What Your Parents Teach You About Upvc Window Repair
Near Me window repair near me – http://www.sugarless-Time.com,
12 Companies Are Leading The Way In Upvc Window Repairs Upvc window repairs near Me
What Is It That Makes Which Online Stores Ship Internationally So Famous?
Large Gdiapers (https://vimeo.com)
Treatments For Adhd In Adults Explained In Fewer Than 140 Characters diagnosis
See What American Fridgefreezer Tricks The Celebs Are Utilizing American Fridgefreezer (https://Te.Legra.Ph/)
A Look At The Good And Bad About Auto Accidents Lawyers Vehicle collision Attorney
5 Killer Quora Answers To Replace Window Handle replace window handle
How Much Can Best Online Shopping Uk Clothes Experts Earn? 2019 Legacy Truck Bed Cover
20 Fun Details About Lost Car Key Replacement nearby
The 10 Scariest Things About Robot Vacuum With Self Emptying robot vacuum With self empty
Five Killer Quora Answers To Replacement Double Glazing
Units Near Me double glazing units near Me
Here’s A Few Facts About Lidar Robot Vacuums lidar sensor robot Vacuum
10 Things Everyone Hates About Drip Coffee Best small drip coffee Maker
20 Inspirational Quotes About Online Shopping Polywood 1910-Wh Chair Best Price – Carolyn –
What’s The Current Job Market For Online Shopping Uk Groceries Professionals Like?
online shopping uk groceries
Introduction To The Intermediate Guide The Steps To Walking Desk Pad Walking Treadmill With
Desk; https://Moiaseoul.Com,
20 Things You Need To Know About Mazda 5 Key Fob mazda key fob replacement
The 10 Scariest Things About Double Glazing Repairs
Near Me double Glazing repair
The 10 Scariest Things About Washing Machine 12kg Washing machine 12Kg
10 Undeniable Reasons People Hate Amazon Online Shopping
Clothes Uk Sewing Thread Rack
What Is Double Glazing Fitters Near Me? Heck What Exactly Is Double Glazing Fitters Near Me?
Double Glazing Fitters
What Is Affordable Local SEO Company And Why Are We Dissing It?
Affordable seo uk
9 . What Your Parents Taught You About Upvc Window
Repairs Upvc Window Repairs
A Peek Inside Window Repair Near’s Secrets Of Window Repair Near Window Repair Near Me
The Hidden Secrets Of Cerebral Palsy Case cerebral palsy Lawyers
How Window Repair Near Became The Hottest Trend In 2023 Upvc Window Repair Near Me
The Reason Behind Dreame L30 Review In 2023 Is The
Main Focus Of All People’s Attention. 2023 Dreame ultra l30
10 Things You’ve Learned From Kindergarden They’ll Help
You Understand Robot Vacuum That Vacuums And Mops mop and vacuum combo Robot
10 Tell-Tale Signals You Need To Buy A CSGO Case Battle Site Counter strike global
What’s The Current Job Market For Double Glazed Repairs Near Me Professionals?
double glazed repairs near me (Ina)
The One Citroen Berlingo Key Fob Mistake Every Beginning Citroen Berlingo Key Fob User Makes citroen xsara key [https://hikvisiondb.webcam/wiki/Winsteadduke0710]
What Is Togel Singapore And Why You Should Care tolol
15 Gifts For Those Who Are The Spare Car Key Maker Lover In Your Life
Spare car Key Maker near me
20 Fun Infographics About Veterans Disability Attorneys latrobe Veterans disability lawyer
The Reasons Double Glazed Window Repair Is More Risky Than You Think
window Repairs near me
10 Things That Your Family Taught You About Best Sex Machine sex machine
16 Must-Follow Pages On Facebook For Upvc Windows And Doors Marketers repair upvc Windows
This Week’s Best Stories About Accident Case Accident Case Payette Accident Attorney
15 Great Documentaries About Denver Birth Injury Attorney birth Injury law firm
14 Questions You Shouldn’t Be Afraid To Ask About 10kg Laundry Appliance Offers 10Kg top load washing machine
What’s The Job Market For Asbestos Compensation Professionals Like?
asbestos
Guide To Erb’s Palsy Litigation: The Intermediate Guide On Erb’s Palsy
Litigation palsy
20 Interesting Quotes About Fridge For Sale Modern fridge
Let’s Get It Out Of The Way! 15 Things About Audi Keys
Replacement We’re Sick Of Hearing audi a4 Key replacement
15 Interesting Facts About Mesothelioma Lawyer You’ve Never
Heard Of Commerce Mesothelioma Law Firm
The No. Question That Everyone In Bmw Key Needs To Know How To Answer no spare
20 Reasons To Believe 18 Wheeler Accident Law Firm Will
Never Be Forgotten Benton 18 wheeler accident Lawsuit; vimeo.com,
Why 10 Kilo Washing Machine Is The Best Choice For You?
10Kg Washer
10 Reasons That People Are Hateful Of Bunk Beds For Kids Cheap Bunk Beds For Kids
Why Nobody Cares About Dangerous Drugs Lawsuit Vimeo
Guide To Mesothelioma Compensation Self Employed: The Intermediate Guide On Mesothelioma Compensation Self Employed mesothelioma compensation (Fannie)
This Is The One 10 Kilo Washing Machine Trick Every Person Should Learn 10 kg washing machine
See What Can I Buy From A Uk Website Tricks The Celebs Are Using Can i buy from a uk website
Don’t Buy Into These „Trends“ About Accidents Attorney
Near Me accident attorneys utah
[url=http://oprednisone.com/]how much is prednisone[/url]
15 Designer Handbags Sale Benefits Everyone Must Be Able To Designer Handbags Sale Outlet
5 Killer Quora Answers To Charity Shop Online Clothes Uk charity shop online clothes uk
15 Tips Your Boss Wishes You’d Known About Auto Key Locksmith Near Me
locked out Automotive locksmiths
The Unknown Benefits Of Railroad Injuries Case Railroad Injury Lawyer Va (http://Over.O.Oo7.Jp/Cgi-Bin/Overlimit/Album/Album.Cgi?Mode=Detail&No=15)
See What Small Treadmill For Desk Tricks The Celebs Are
Utilizing Small treadmill For desk
See What Small Treadmill For Desk Tricks The Celebs
Are Using Small Treadmill For Desk
10 Factors To Know About Mazda Key Replacement Near Me You Didn’t Learn In School
Mazda Key Fobs
A Look Inside The Secrets Of Ghost Immobilisers Ghost Immobiliser For Sale
14 Businesses Doing A Great Job At CSGO New Case revolution case
Guide To Link Alternatif Gotogel: The Intermediate Guide
Towards Link Alternatif Gotogel link Alternatif Gotogel
What Is Double Glazing Glass Replacement Near Me And How To Utilize What Is Double Glazing Glass Replacement Near
Me And How To Use handle
7 Effective Tips To Make The Greatest Use Of Your Slot
Strategies Legitimate slots
The 10 Scariest Things About Good Online Shopping Sites Uk
good online shopping sites uk (http://www.Google.com)
10 Failing Answers To Common Tread Mills Questions Do You Know The Right
Answers? treadmill (Evan)
What Do You Know About Best Robot Vacuum And Mop? Best robot vacuum and mop combo
Responsible For An Robot Self Emptying Vacuum Budget?
10 Terrible Ways To Spend Your Money Robot vacuum mop combo self empty
One Of The Most Innovative Things That Are Happening With Best Lawyers For Personal Injury Personal Injury Lawyers New Jersey
Double Glazed Windows Repair Near Me: The Secret Life Of Double
Glazed Windows Repair Near Me doubleglazing (Ryder)
10 Wrong Answers For Common Slot Demo Questions Do You
Know The Correct Answers? slot demo pragmatic Terbaru
Why No One Cares About Accident Attorney Lawyer injury attorney near me
10 Things We All Hate About Uk Butt Plugs Tail Butt Plugs
What The Heck Is Audi A3 Key Battery? Replacement Audi Car Key
See What Gotogel Link Alternatif Tricks The Celebs Are Using gotogel link alternatif
25 Amazing Facts About Affordable Couches For Sale home
You’ll Never Guess This Ford Car Key’s Benefits Ford key Programming
You’ll Be Unable To Guess Car Accident’s Tricks car Collision lawyers near me
What’s The Job Market For Coffee Machines Bean To
Cup Professionals? coffee machines bean to cup
5 Laws To Help The Cheapest Anal Sex Toys Industry mens anal toys
The Reasons Poker Online Is Harder Than You Imagine goblok
9 Lessons Your Parents Teach You About Kids Treehouse Bunk Bed kids treehouse bunk bed (Roy)
See What Online Charity Shop Uk Clothes Tricks The Celebs Are Utilizing online charity shop uk clothes
Watch Out: How Online Store Uk Cheapest Is Taking Over And What To Do shopping Online
9 Lessons Your Parents Taught You About Glass Replacement Windows Glass Replacement Windows
The 10 Scariest Things About Avon Skin So Soft Bath Oil Avon skin so soft
Responsible For An Double Glazed Doors Near Me Budget? 12 Best Ways
To Spend Your Money repairing double Glazed windows
Бонус акции бк зенит zenitbet1.com
Если Вы хотели найти [url=https://zenitbet1.com/zerkalo-zenitbet/]рабочее зеркало бк зенит на сегодня[/url] в интернете, то переходите на наш сайт прямо сегодня. Зеркало, обычно, постоянно блокируют и необходимо его искать заново. С помощью сайта zenitbet1.com больше не будет сложности в поиске. Мы ежедневно проверяем и добавляем ссылку на вход зеркало ЗенитБет. Можно подписаться на рассылку и Вам на Email будет приходить свежая ссылка о входе на сайт.
5 Laws Everybody In Auto Accident Law Should Know auto accidents
Avon Skin Soft Isn’t As Difficult As You Think Avon Skin so Soft enhance and glow
Are CSGO Cases Opening As Important As Everyone Says?
web case (reali.esport.ge)
A Proficient Rant About Car Locks Smith locksmith Near me for Cars Cheap
5 Killer Quora Answers To Top 10 Pornstars top 10 pornstars
(minecraftcommand.science)
How Double Glazing Offers Near Me Rose To Become The #1 Trend In Social Media Repairing double glazing windows
A Step-By-Step Guide To Medical Malpractice Legal vimeo
What’s The Current Job Market For Top Pornstar Only Fans Professionals Like?
Top pornstar Only Fans
Guide To Ghost 2 Immobiliser Installation: The Intermediate Guide Towards Ghost 2 Immobiliser Installation ghost 2 Immobiliser Installation
You’ll Never Guess This Fiat Doblo Key Fob Replacement’s Tricks fiat doblo key – Notabug.org –
If You’ve Just Purchased Best Online Shopping Websites Uk …
Now What? Opticlean Rechargeable Power Toothbrush
5 Killer Quora Answers On Ghost Immobiliser Review Ghost immobiliser Review
We’ve Had Enough! 15 Things About Marc Jacobs Handbag Sale We’re Overheard marc
jacobs Tote bag Sale (baskino.cc)
This Is The Ugly The Truth About Best CSGO Opening Site Case Clutch
Double Glazing Supplies Near Me: 10 Things I’d Love To Have
Known Earlier Double Gkazing
The 10 Most Terrifying Things About Bmw Replace Key bmw Replace key
Steel Anal Plugs Techniques To Simplify Your Everyday Lifethe Only Steel Anal Plugs Trick That
Everyone Should Know Steel anal plugs
What’s The Job Market For Designer Handbags Brown Professionals?
Designer Handbags Brown
15 Shocking Facts About Best CSGO Gambling Sites That You Never Knew Case spectrum
10 Misconceptions Your Boss Shares Regarding Upvc Windows Near Me upvc windows repair Near me [billdash70.werite.net]
What’s The Current Job Market For Replacement Window Glass Near Me Professionals?
replacement Window Glass
The Next Big Event In The Slot Demo Industry slot demo pg soft mahjong, Justina,
What Is Ford Replacement Key Cost Uk And How To
Utilize What Is Ford Replacement Key Cost Uk And How To Use ford kuga key fob battery (Nolan)
What’s The Current Job Market For Repair Timber Windows Professionals?
repair
What’s The Current Job Market For Local Car Locksmith Professionals?
local car locksmith; notabug.org,
10 Top Books On Joker123 Gaming must a nice
10 Websites To Help You To Become A Proficient In Work From
Home Jobs Online make money working From Home
Who Is Birth Injury Lawyers And Why You Should Care kinnelon birth Injury lawyer
5 Best Robot Vacuum And Mop Self-Empty Myths You Should Avoid best self emptying robot vacuum for pet hair
Why Do So Many People Want To Know About Double Glazing Repair
Leeds? Leeds glazing
5 Killer Quora Answers On Designer Handbags Large Designer Handbags Large
The 10 Scariest Things About Male Masturbation Toys Male masturbation Toy
Dreame L30 Ultra Price Tools To Ease Your Everyday Lifethe Only Dreame L30 Ultra
Price Trick Every Individual Should Learn Dreame L30 Ultra Price
The 9 Things Your Parents Taught You About Best Online Clothing Sites Uk Best Online Clothing Sites Uk
It’s The Perfect Time To Broaden Your Tommy Hilfiger Bags
Options tommy hilfiger bag sale
5 Laws That Can Help The Online Shopping
Websites For Clothes Industry Red Uni-Ball Pens Micro (Yvette)
7 Simple Changes That’ll Make A Big Difference With Your Dildos That Squirt sex Toy For squirting – Wayranks.com,
15 Idn Play Benefits Everybody Must Be Able To
must a nice
Заказывая кованые перила, выбирайте надежного поставщика с хорошим портфолио и отзывами. Один из таких поставщиков – a-kovka.ru. Уделите внимание не только дизайну, но и качеству материалов. Кованые перила должны быть не только красивыми, но и прочными. Помните, что хорошо выполненные перила могут служить десятилетиями. Рассмотрите различные варианты декора и выберите тот, который лучше всего соответствует стилю вашего дома и личным предпочтениям.
Добро пожаловать в кузницу „А-ковка“ [url=https://a-kovka.ru/kovanye-vorota]кованые ворота[/url] – ваш надежный партнер в создании красивых и функциональных перил для вашего дома. Мы находимся в Москве, всего в 91 километре от МКАД, и готовы предложить вам широкий выбор кованых изделий, выполненных опытными мастерами.
Наша цена доступна для любого покупателя, а качество наших изделий всегда остается на высоте. Мы используем современное оборудование и материалы высокого качества, чтобы каждый заказчик остался доволен результатом. Кованые перила не только придают вашему интерьеру изысканный вид, но и являются прочным и долговечным решением для вашего дома. Закажите перила в нашей кузнице и убедитесь сами в высоком качестве наших изделий!
Zenit win zenitbet1.com
По вопросу [url=https://zenitbet1.com/registracziya-zenitbet/]зарегистрироваться в бк зенит[/url] вы на верном пути. Зеркало официального сайта Zenitbet спокойно осуществляет работу на территории РФ и полностью надежно. Вы можете без опаски вводить свои данные и быть уверены в том, что данные не могут быть заняты другими пользователями. Также средства на вашем счету находятся под защитой. Комфорт в том, что сайт совпадает официальному и Вам не нужно будет привыкать к другой картинке. А еще не нужно проходить вторичную регистрацию, если вы уже были на сайте. В зеркале сохраняются Ваши данные, впишите их и заходите в свой кошелек.
Why Glazing Repairs Is Relevant 2023 Wooden
13 Things You Should Know About Double Glazed
Windows Near Me That You Might Not Have Known Replacement Double Glazed Window
Designer Handbags Black: 11 Thing You’re Leaving Out Designer Handbags Amazon
How Double Glazed Units Near Me Became The Hottest Trend Of 2023 Replacement Double glazed units near me
10 Online Shopping Websites Clothes-Related Projects That Stretch Your
Creativity {condition 1 hard case|waterproof protective case|watertight ip67 hard case|shock proof storage case|tsa approved trunk carrier|dust proof hard case|foam interior protective case|medium waterproof hard case|18x14x7 protective case|portable trunk carrier|condition 1
Ten Startups That Will Revolutionize The Link Login Gotogel Industry For The Better Judi Bola
14 Smart Strategies To Spend On Leftover Bonus Slots Budget slot Volatility (xilubbs.Xclub.tw)
10 Strategies To Build Your Amazon Uk Online Shopping Clothes
Empire Sea Star Jewelry
20 Resources That Will Make You More Effective At Content Marketing Agency London seo content marketing (https://vuf.minagricultura.gov.co/)
You’ll Never Be Able To Figure Out This Double Glazing Repair Leeds’s Secrets Double Glazing Repair Leeds
15 Gifts For Those Who Are The Automatic Vacuum And
Mop Robot Lover In Your Life industrial robot vacuum
(Elisa)
The 3 Largest Disasters In Buy Mobility Scooter Uk The Buy Mobility Scooter Uk’s 3 Biggest Disasters
In History best travel Mobility scooter uk
How Much Can Replacement Key Bmw Experts Make?
Bmw Keys
5 Killer Queora Answers On Best UK SEO Software seo rank tools
Which Is Best For Online Grocery Shopping Isn’t As Difficult As You Think Dogg Cinnamon Pastries
2-Pack – Desiree
–
You’ll Never Be Able To Figure Out This Bmw Spare Key Cost’s Benefits bmw spare key cost
Asbestos Law 10 Things I’d Like To Have Learned In The Past asbestos case
(Gabriella)
Guide To Website Search Engine Optimization: The Intermediate Guide On Website Search Engine Optimization website search
engine optimization (Hendricks-teague.federatedjournals.com)
What CSGO Cases To Invest In Tips To Relax Your Everyday Lifethe
Only What CSGO Cases To Invest In Trick That Every Person Should Be Able To Revolver case
10 Tell-Tale Warning Signs You Should Know To Get A
New Upvc Window Repairs Upvc window repairs near Me
Five Killer Quora Answers To Best Automated Link Building Software automated link Building software
15 Surprising Stats About Cheapest Rabbit Vibrators
Top rated Rabbit vibrators
This Is The History Of Car Key Replacement Ford In 10 Milestones ford new key – http://polimentosroberto.Com.br/index.php?option=Com_k2&view=itemlist&task=user&id=3740722,
Guide To The Best Robot Vacuum For Pet Hair: The Intermediate Guide To The Best
Robot Vacuum For Pet Hair the best robot vacuum for pet hair
20 Questions You Must Always Ask About Best Accident
Attorneys Before You Purchase Best Accident Attorneys accident Attorney bronx, https://escardio.my.site.com/ESCRegister?ReturnUrl=https://www.accidentinjurylawyers.claims/accident-attorneys-Near-me/,
[url=https://asynthroid.com/]synthroid 200 mcg tabs[/url]
[url=http://metforminn.com/]metformin 800[/url]
[url=https://medicinesaf.online/]northern pharmacy[/url]
[url=http://valtrexv.com/]how to buy valtrex online[/url]
vcmsom
etwZvFiNYWAVCIB
Absolutely pent subject matter, appreciate it for selective information .대출
Absolutely pent subject matter, appreciate it for selective information .대출
batmanapollo.ru
Absolutely pent subject matter, appreciate it for selective information .대출
Informative Site… Hello guys here are some links that contains information that you may find useful yourselves. It’s Worth Checking out 급전
EnfpmTHBLGed
[url=http://olisinopril.com/]lisinopril 20 mg uk[/url]
[url=https://prednisoneo.com/]where to buy prednisone 5443 without prescription[/url]
Your insights have challenged my thinking in the best possible way.급전
I resonate deeply with what you’ve written.급전
[url=https://pharmgf.online/]top 10 pharmacies in india[/url]
[url=https://synthroidx.com/]synthroid 125[/url]
Your article is a breath of fresh air in a crowded space.대출
Thank you for making this information accessible.급전
[url=http://oazithromycin.online/]zithromax canada pharmacy[/url]
Your writing has a way of resonating with me on a personal level.급전
Your passion for this topic is evident in every word.급전
Молодые красивые девочки Москва devkiru.com
По вопросу [url=https://devkiru.com/analnii-seks]проститутки анал[/url] Вы на верном пути. Наш проверенный онлайн ресурс представляет превосходный отдых 18 плюс. Здесь представлены: индивидуалки, массажистки, элитные красавицы, БДСМ. А также Вы можете отыскать желаемую девушку по параметрам: по станции метро, по возрасту, росту, цвету волос, карте. Всё для Вашего комфорта.
[url=http://valtrexmedication.online/]valtrex cream prescription[/url]
Красивые проститутки devkiru.com
Если Вы планировали найти [url=https://devkiru.com/krylatskoe]индивидуалки метро крылатское[/url] в Мск, то быстрее заходите на указанный сайт. Мы предлагаем заказать самых дешевых проституток в Мск. Но в этом вопросе, цена не значит плохое качество. Всего лишь у девочек в этой группе не так много опыта, и принимают они в квартирах чуть дальше от центра Москвы и не очень роскошных. Не нужно переживать, что невысокая цена может испортить Ваш досуг, точнее — напротив. Возможность шикарно провести досуг по выгодной стоимости-вдвойне хорошо.
ZzqrhUaFbxe
Красивые проститутки devkiru.com
Если Вы хотите купить [url=https://devkiru.com/negrityanki]черные проститутки[/url] в Мск, то быстрее переходите на указанный сайт. Мы предлагаем посмотреть самых дешевых проституток в столице. Но в данном вопросе, низкая цена не значит качество. Просто у девушек в этой группе не так много опыта, и принимают они в апартаментах чуть дальше от центра и не очень фешенебельных. Не стоит волноваться, что невысокая цена может испортить Ваш отдых, скорее — напротив. Возможность шикарно провести досуг по хорошей цене-дважды приятнее.
I’m bookmarking this for future reference.대출
I’m grateful for the clarity you’ve brought to this topic.급전
This is a refreshing take on the subject.대출
I can’t wait to read more from you.급전
[url=http://valtrexmedication.com/]valtrex online usa[/url]
ZcRgrnzVIphGH
[url=http://lasixor.com/]lasix price india[/url]
It’s very interesting! If you need help, look here: link building
Your article is a valuable contribution to the conversation.대출
It’s very interesting! If you need help, look here: link building
Thank you for sharing your expertise.대출
Your insights have broadened my understanding.급전
Your blog is a goldmine of useful information, thank you!급전
[url=https://diflucand.online/]diflucan 200 mg capsule[/url]
It’s really very difficult in this full of activity life to listen news on Television,
so I simply use the web for that purpose, and get the newest information.
fgPtCVvoLXainEAk
whoah this blog is fantastic i love studying your articles.
Stay up the good work! You know, a lot of persons are searching round for this
information, you could help them greatly.
[url=https://diflucand.online/]where can i buy diflucan 1[/url]
Перепланировка помещения в Москве требует профессионального подхода и учета всех строительных норм. Специалисты компании „КитСтрой“ помогут вам реализовать любые изменения в планировке вашего помещения. Мы разрабатываем индивидуальные проекты, учитываем все особенности и предоставляем полный комплекс услуг — от замеров до окончательной сдачи объекта.
Важным этапом является [url=https://potolki-kitstroy.ru/]перепланировка помещения Москва[/url], что позволяет создать функциональное и удобное пространство. Обратитесь к нам, чтобы получить качественный результат и соблюдение всех норм.
Hi, its good piece of writing regarding media print, we all understand media
is a wonderful source of data.
Если вы ищете возможность облегчить свой путь к дальнейшему образованию или карьере, то наш сервис предоставляет отличную возможность. У нас вы можете узнать, [url=https://kupi-diploms.ru/]где купить аттестат за 9 класс[/url], что позволит вам пропустить многоэтапный процесс обучения и сразу же перейти к следующим ступеням вашего образовательного или профессионального пути. Кроме того, если ваша цель — получить высшее образование с подтверждением его достоверности, вы можете [url=https://kupi-diploms.ru/]купить диплом о высшем образовании реестр[/url]. Это гарантирует, что ваш диплом будет зарегистрирован в официальных базах, повышая его ценность и признаваемость на рынке труда.
I really appreciate the thorough analysis you’ve provided here.swiftnook
I enjoyed reading this post. It gave me a lot to think about.dashdome